lb_luria_bertani_agar_with_5_nacl_ph_9_0

CultureMech:002356 bacterial COMPLEX SOLID_AGAR
Original name: LB (LURIA-BERTANI) AGAR WITH 5% NaCl (pH 9.0)

Overview

External ontology
mediadive.medium:J1185 (source link unavailable) — LB (LURIA-BERTANI) AGAR WITH 5% NaCl (pH 9.0)
Applications
  • Microbial cultivation
pH
9.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1185

Composition

flowchart LR
    CultureMech_002356["lb_luria_bertani_agar_with_5_nacl_ph_9_0"]
    ing0_Tryptone["Tryptone"]
    CultureMech_002356 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002356 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_002356 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Agar["Agar"]
    CultureMech_002356 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Tryptone 10 G_PER_L
2 Yeast extract 5 G_PER_L
3 NaCl
Cl.Na 58.443 g/mol
50 G_PER_L CHEBI:26710
sodium chloride
4 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. After autoclaving, adjust pH to 9.0 with sterilized 10% Na2CO3 solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.940904Z mediadive-import Imported from MediaDive Source: JCM, ID: J1185
2026-03-13T06:19:12.662722Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002356
2026-03-17T06:30:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.675568+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB (LURIA-BERTANI) AGAR WITH 5% NaCl (pH 9.0)" to "lb_luria_bertani_agar_with_5_nacl_ph_9_0" for consistent matching
2026-06-05T07:41:10.890164+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T15:40:13.763859+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.