Overview
- External ontology
- TOGO:M3279 — Alkaline Diluted AMS Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3279 Original source: JCM - JCM_M1428 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1428
Composition
flowchart LR
CultureMech_009693["alkaline_diluted_ams_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009693 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009693 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009693 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_009693 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Methane_gas["Methane gas"]
CultureMech_009693 --> ing4_Methane_gas
ing5_Air["Air"]
CultureMech_009693 --> ing5_Air
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 5 | Methane gas | variable VARIABLE | Properties: ガス | |
| 6 | Air | variable VARIABLE | Properties: ガス |
Solutions
Trace element solution (see Medium [M850])
Dose: 0.1 G_PER_L
Cross-reference to Medium M850
Iron stock solution (see Medium [M850])
Dose: 0.1 G_PER_L
Cross-reference to Medium M850
Phosphate buffer stock solution (see Medium [M850])
Dose: 1 G_PER_L
Cross-reference to Medium M850
1 N HCl solution
Dose: variable VARIABLE
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- alkaline_diluted_ams_medium (CultureMech:009693)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.720776Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M3279 |
| 2026-01-28T19:48:32.859245Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 1 N HCl solution; Added default concentration for ingredient: Methane gas; Added default concentration for ingredient: Air |
| 2026-03-09T08:04:54.366378+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:20:08.190259Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009693 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.179419+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkaline Diluted AMS Medium" to "alkaline_diluted_ams_medium" for consistent matching |
| 2026-05-16T09:03:23.626334+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:47.860586+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:35.541294+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.791228+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.357409+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint cbbf40f371ee682861d4a987cae6349ff991d561663770fd71ea1e93217bdb6a |