Overview
- External ontology
- TOGO:M3279 — Alkaline Diluted AMS Medium
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- TOGO M3279 records JCM Medium 1428, which uses JCM Medium 815 with the final pH adjusted to 8.0, the phosphate buffer stock reduced to 1.0 ml/L, and the gas phase replaced by methane-air (80:20, v/v). JCM Medium 815 supplies the basal NH4Cl, MgSO4 x 7 H2O, CaCl2 x 2 H2O, Trace element solution, Iron stock solution, and Phosphate buffer stock solution formulas.
Composition
flowchart LR
CultureMech_009693["alkaline_diluted_ams_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_009693 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009693 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009693 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_Distilled_water["Distilled water"]
CultureMech_009693 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.1 G_PER_L |
CHEBI:31206
ammonium chloride
|
JCM Medium 1428 uses the JCM Medium 815 basal component NH4Cl at 0.1 g/L. |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
JCM Medium 1428 uses the JCM Medium 815 basal component MgSO4 x 7 H2O at 0.1 g/L. |
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
JCM Medium 1428 uses the JCM Medium 815 basal component CaCl2 x 2 H2O at 0.02 g/L. |
| 4 |
Distilled water
H2O 18.015 g/mol
|
1000.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 1428 uses the JCM Medium 815 basal component Distilled water at 1000.0 ml/L. |
Preparation
- Dissolve NH4Cl, MgSO4 x 7 H2O, and CaCl2 x 2 H2O in distilled water with 0.1 ml/L Trace element solution and 0.1 ml/L Iron stock solution.
- Autoclave the basal medium.
- Autoclave the Phosphate buffer stock solution separately.
- After cooling, add 1.0 ml/L sterile Phosphate buffer stock solution to the basal medium.
- Adjust the medium to pH 8.0.
- Distribute 20 ml aliquots into sterile 120 ml serum bottles and seal with butyl rubber stoppers.
- Replace the gas phase with filter-sterilized methane-air (80:20, v/v).
Solutions
Trace element solution
Dose: 0.1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| ZnSO4 x 7 H2O | 0.4 G_PER_L | JCM Medium 815 lists 0.4 g/L ZnSO4 x 7 H2O in this stock solution. |
| EDTA x 2Na | 0.25 G_PER_L | JCM Medium 815 lists 0.25 g/L EDTA x 2Na in this stock solution. |
| MnCl2 x 4 H2O | 0.02 G_PER_L | JCM Medium 815 lists 0.02 g/L MnCl2 x 4 H2O in this stock solution. |
| H3BO3 | 0.015 G_PER_L | JCM Medium 815 lists 0.015 g/L H3BO3 in this stock solution. |
| Na2MoO4 x 2 H2O | 0.04 G_PER_L | JCM Medium 815 lists 0.04 g/L Na2MoO4 x 2 H2O in this stock solution. |
| NiCl2 x 6 H2O | 0.01 G_PER_L | JCM Medium 815 lists 0.01 g/L NiCl2 x 6 H2O in this stock solution. |
| CuSO4 x 5 H2O | 0.2 G_PER_L | JCM Medium 815 lists 0.2 g/L CuSO4 x 5 H2O in this stock solution. |
| CoCl2 x 6 H2O | 0.05 G_PER_L | JCM Medium 815 lists 0.05 g/L CoCl2 x 6 H2O in this stock solution. |
| Distilled water | 1.0 L | JCM Medium 815 lists 1.0 L Distilled water in this stock solution. |
JCM Medium 1428 uses the JCM Medium 815 0.1 ml/L Trace element solution without modifying its composition.
Iron stock solution
Dose: 0.1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| EDTA x Fe(III) | 4.5 G_PER_L | JCM Medium 815 lists 4.5 g/L EDTA x Fe(III) in this stock solution. |
| Distilled water | 1.0 L | JCM Medium 815 lists 1.0 L Distilled water in this stock solution. |
JCM Medium 1428 uses the JCM Medium 815 0.1 ml/L Iron stock solution without modifying its composition.
Phosphate buffer stock solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| KH2PO4 | 37.425 G_PER_L | JCM Medium 815 lists 37.425 g/L KH2PO4 in this stock solution. |
| Na2HPO4 x 2 H2O | 48.950 G_PER_L | JCM Medium 815 lists 48.950 g/L Na2HPO4 x 2 H2O in this stock solution. |
| Distilled water | 1.0 L | JCM Medium 815 lists 1.0 L Distilled water in this stock solution. |
JCM Medium 1428 reduces the JCM Medium 815 Phosphate buffer stock solution addition to 1.0 ml/L.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.720776Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M3279 |
| 2026-01-28T19:48:32.859245Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 1 N HCl solution; Added default concentration for ingredient: Methane gas; Added default concentration for ingredient: Air |
| 2026-03-09T08:04:54.366378+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:20:08.190259Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009693 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.179419+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkaline Diluted AMS Medium" to "alkaline_diluted_ams_medium" for consistent matching |
| 2026-05-16T09:03:23.626334+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:47.860586+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:35.541294+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.791228+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m3279_alkaline_diluted_ams_score15.py | RESOLVED_TOGO_M3279_ALKALINE_DILUTED_AMS | Verified TOGO M3279 against JCM Medium 1428 and its JCM Medium 815 parent, corrected water to 1000.0 ml/L, converted imported JCM 815 stock references to 0.1 ml/L Trace element solution, 0.1 ml/L Iron stock solution, and 1.0 ml/L Phosphate buffer stock solution, moved the methane-air gas phase out of ingredients, and removed the JCM 815 pH 3.0 HCl adjustment. |