Overview
- External ontology
- TOGO:M3282 — RCM Broth
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3282 Original source: JCM - JCM_M1446 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1446
Composition
flowchart LR
CultureMech_009696["rcm_broth"]
ing0_Distilled_water["Distilled water"]
CultureMech_009696 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009696 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Sodium_acetate["Sodium acetate"]
CultureMech_009696 --> ing2_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing2_Sodium_acetate -.-> CHEBI_32954
ing3_Glucosev["Glucosev"]
CultureMech_009696 --> ing3_Glucosev
ing4_Soluble_starch["Soluble starch"]
CultureMech_009696 --> ing4_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing4_Soluble_starch -.-> CHEBI_28017
ing5_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009696 --> ing5_Yeast_extract__BD_Difco_
ing6_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_009696 --> ing6_Bacto_peptone__BD_Difco_
ing7_L_Cysteine_HCl_H2O["L-Cysteine·HCl·H2O"]
CultureMech_009696 --> ing7_L_Cysteine_HCl_H2O
ing8_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
CultureMech_009696 --> ing8_Beef_extract__BD_Difco_
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
3 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 4 | Glucosev | 5 G_PER_L | Role: Carbon source; Properties: Simple component, Organic compound, Defined component | |
| 5 | Soluble starch | 1 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 6 | Yeast extract (BD-Difco) | 3 G_PER_L | Properties: Undefined component | |
| 7 | Bacto peptone (BD-Difco) | 10 G_PER_L | Properties: Undefined component | |
| 8 | L-Cysteine·HCl·H2O | 0.5 G_PER_L | Properties: Organic compound, Simple component, Defined component | |
| 9 | Beef extract (BD-Difco) | 10 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- rcm_broth (CultureMech:009696)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.724015Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M3282 |
| 2026-01-28T19:48:42.298713Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:08.211556Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009696 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.200172+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RCM Broth" to "rcm_broth" for consistent matching |
| 2026-06-05T07:41:43.900620+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:54.360334+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bdb25a0bae869314b74aabf60cbae6c567131afd27300ceddb813e211efafa24 |