rcm_broth (CultureMech:009696)

Overview

External ontology

TOGO:M3282 — RCM Broth

Applications

Microbial cultivation

pH

6.8

Notes

TOGO M3282 imports JCM Medium 1446 RCM Broth, which lists, per liter, 10 g Bacto peptone (BD-Difco), 10 g Beef extract (BD-Difco), 3 g Yeast extract (BD-Difco), 5 g Glucose, 5 g NaCl, 1 g Soluble starch, 0.5 g L-Cysteine HCl H2O, 3 g Sodium acetate, 1 L distilled water, final pH 6.8, and the JCM default autoclaving at 121 C for 15 min.

Composition

flowchart LR
    CultureMech_009696["rcm_broth"]
    ing0_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
    CultureMech_009696 --> ing0_Bacto_peptone__BD_Difco_
    ing1_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
    CultureMech_009696 --> ing1_Beef_extract__BD_Difco_
    ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009696 --> ing2_Yeast_extract__BD_Difco_
    ing3_Glucose["Glucose"]
    CultureMech_009696 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_NaCl["NaCl"]
    CultureMech_009696 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Soluble_starch["Soluble starch"]
    CultureMech_009696 --> ing5_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing5_Soluble_starch -.-> CHEBI_28017
    ing6_L_Cysteine_HCl_H2O["L-Cysteine·HCl·H2O"]
    CultureMech_009696 --> ing6_L_Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_H2O -.-> CHEBI_91248
    ing7_Sodium_acetate["Sodium acetate"]
    CultureMech_009696 --> ing7_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Sodium_acetate -.-> CHEBI_32954
    ing8_Distilled_water["Distilled water"]
    CultureMech_009696 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	Bacto peptone (BD-Difco)	10.0 G_PER_L	MICRO:0000178 (source link unavailable) Peptone	JCM Medium 1446 lists 10.0 g/L Bacto peptone (BD-Difco).
2	Beef extract (BD-Difco)	10.0 G_PER_L	FOODON:03302088 beef extract	JCM Medium 1446 lists 10.0 g/L Beef extract (BD-Difco).
3	Yeast extract (BD-Difco)	3.0 G_PER_L	FOODON:03315426 yeast extract	JCM Medium 1446 lists 3.0 g/L Yeast extract (BD-Difco).
4	Glucose C6H12O6 180.156 g/mol	5.0 G_PER_L	CHEBI:17234 glucose	JCM Medium 1446 labels this row as Glucosev; TOGO M3282 normalizes the component as Glucose.
5	NaCl Cl.Na 58.443 g/mol	5.0 G_PER_L	CHEBI:26710 sodium chloride	JCM Medium 1446 lists 5.0 g/L NaCl.
6	Soluble starch	1.0 G_PER_L	CHEBI:28017 starch	JCM Medium 1446 lists 1.0 g/L Soluble starch.
7	L-Cysteine·HCl·H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.5 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	JCM Medium 1446 lists 0.5 g/L L-Cysteine·HCl·H2O.
8	Sodium acetate C2H3O2.Na 82.034 g/mol	3.0 G_PER_L	CHEBI:32954 sodium acetate	JCM Medium 1446 lists 3.0 g/L Sodium acetate.
9	Distilled water H2O 18.015 g/mol	1.0 L	CHEBI:15377 water	JCM Medium 1446 lists 1.0 L Distilled water.

Preparation

Dissolve Bacto peptone, beef extract, yeast extract, glucose, NaCl, soluble starch, L-cysteine hydrochloride monohydrate, and sodium acetate in 1.0 L distilled water.
Adjust pH to 6.8.
Autoclave at 121 C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:53.724015Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M3282
2026-01-28T19:48:42.298713Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:08.211556Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009696
2026-03-17T06:30:55.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.200172+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "RCM Broth" to "rcm_broth" for consistent matching
2026-06-05T07:41:43.900620+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00	repair_togo_m3282_rcm_broth_score15.py	RESOLVED_TOGO_M3282_RCM_BROTH_SCORE15	Corrected the imported distilled-water unit from g/L to L, normalized the Glucosev source typo, added pH 6.8 and the JCM autoclave step, grounded all nine disclosed components, and marked the JCM formula as curated.