isp_4_plus_20_nacl

CultureMech:009704 bacterial COMPLEX SOLID_AGAR
Original name: ISP 4 plus 20% NaCl

Overview

External ontology
TOGO:M3291 — ISP 4 plus 20% NaCl
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'inorganic_salt_solution_agar_isp_medium_no_4_10_nacl', 'source': 'TOGO', 'source_id': 'TOGO:M1963', 'original_category': 'bacterial'}
  • {'name': 'inorganic_salts_starch_agar_isp_medium_no_4', 'source': 'TOGO', 'source_id': 'TOGO:M1486', 'original_category': 'bacterial'}
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M3291 Original source: NBRC - NBRC_M1661 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1661

Composition

flowchart LR
    CultureMech_009704["isp_4_plus_20_nacl"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009704 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_009704 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_009704 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4__anhydrous_base_["K2HPO4 (anhydrous base)"]
    CultureMech_009704 --> ing3_K2HPO4__anhydrous_base_
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009704 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_CaCO3["CaCO3"]
    CultureMech_009704 --> ing5_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing5_CaCO3 -.-> CHEBI_3311
    ing6_Soluble_starch["Soluble starch"]
    CultureMech_009704 --> ing6_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing6_Soluble_starch -.-> CHEBI_28017
    ing7_Bacto_Agar__Difco_["Bacto Agar (Difco)"]
    CultureMech_009704 --> ing7_Bacto_Agar__Difco_
    ing8_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_009704 --> ing8_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_7H2O -.-> CHEBI_75836
    ing9_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_009704 --> ing9_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_4H2O -.-> CHEBI_86368
    ing10_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_009704 --> ing10_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_7H2O -.-> CHEBI_32312

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
101.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 1.0, 100.0]
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 K2HPO4 (anhydrous base) 1 G_PER_L Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
6 CaCO3
CO3.Ca 100.086 g/mol
2 G_PER_L CHEBI:3311
calcium carbonate
 Role: Protective agent, Mineral source; Properties: Defined component, Simple component, Inorganic compound
7 Soluble starch 10 G_PER_L CHEBI:28017
starch
 Role: Carbon source; Properties: Undefined component
8 Bacto Agar (Difco) 20 G_PER_L Role: Solidifying component
9 FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
11 ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component

Solutions

Trace salts solution*

Dose: 1 G_PER_L

See mediadive_3683_Trace_salts_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:59.267068Z togo-import Imported from TOGO Medium Source: NBRC, ID: M3291
2026-01-28T19:48:31.302800Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:52.149828+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:09:23.535292Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:08.270810Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009704
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.261299+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ISP 4 plus 20% NaCl" to "isp_4_plus_20_nacl" for consistent matching
2026-05-16T09:03:38.010569+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:09.679980+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:49.602478+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:00.147425+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:38.424718+00:00 recipe-merger Merged 3 duplicate recipes into canonical record Sources: inorganic_salt_solution_agar_isp_medium_no_4_10_nacl, inorganic_salts_starch_agar_isp_medium_no_4, isp_4_plus_20_nacl
2026-08-06T03:05:38.424843+00:00 merge_recipes.py MERGED_RECIPES merged 3 source recipe(s) on fingerprint 2d493e79ced8a444b1c8c6833a742ab42c30a527fab5f14e31c66a780eb9a0aa