Overview
- External ontology
- TOGO:M1963 — Inorganic Salt Solution Agar (ISP Medium No. 4) + 10% NaCl
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1963 Original source: NBRC - NBRC_M1240 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1240
Composition
flowchart LR
CultureMech_008544["inorganic_salt_solution_agar_isp_medium_no_4_10_nacl"]
ing0_Distilled_water["Distilled water"]
CultureMech_008544 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008544 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_008544 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4__anhydrous_base_["K2HPO4 (anhydrous base)"]
CultureMech_008544 --> ing3_K2HPO4__anhydrous_base_
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_008544 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_CaCO3["CaCO3"]
CultureMech_008544 --> ing5_CaCO3
CHEBI_3311["CHEBI:3311"]
ing5_CaCO3 -.-> CHEBI_3311
ing6_Soluble_starch["Soluble starch"]
CultureMech_008544 --> ing6_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing6_Soluble_starch -.-> CHEBI_28017
ing7_Bacto_Agar__Difco_["Bacto Agar (Difco)"]
CultureMech_008544 --> ing7_Bacto_Agar__Difco_
ing8_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008544 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
ing9_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008544 --> ing9_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_4H2O -.-> CHEBI_86368
ing10_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008544 --> ing10_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_7H2O -.-> CHEBI_32312
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
101.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 1.0, 100.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
100 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 | K2HPO4 (anhydrous base) | 1 G_PER_L | Role: Buffer; Properties: Defined component, Simple component, Inorganic compound | |
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
CaCO3
CO3.Ca 100.086 g/mol
|
2 G_PER_L |
CHEBI:3311
calcium carbonate
|
Role: Protective agent, Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 | Soluble starch | 10 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 8 | Bacto Agar (Difco) | 20 G_PER_L | Role: Solidifying component | |
| 9 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 11 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
Solutions
Trace salts solution*
Dose: 1 G_PER_L
See mediadive_3683_Trace_salts_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.748353Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1963 |
| 2026-01-28T19:48:14.863856Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:24.730856+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:53.165454Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:57.897734Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008544 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.524391+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Inorganic Salt Solution Agar (ISP Medium No. 4) + 10% NaCl" to "inorganic_salt_solution_agar_isp_medium_no_4_10_nacl" for consistent matching |
| 2026-05-16T09:03:37.784801+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:09.378442+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:49.395856+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:00.048846+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |