Overview
- External ontology
- TOGO:M3294 — HM Buffer
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3294 Original source: NBRC - NBRC_M1663 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1663
Composition
flowchart LR
CultureMech_009705["hm_buffer"]
ing0_HEPES["HEPES"]
CultureMech_009705 --> ing0_HEPES
CHEBI_46756["CHEBI:46756"]
ing0_HEPES -.-> CHEBI_46756
ing1_UPW["UPW"]
CultureMech_009705 --> ing1_UPW
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | HEPES | 5 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 2 | UPW | 100 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
Solutions
CaCl2 solution
Dose: 600 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
MgCl2 solution
Dose: 333 G_PER_L
Role: Mineral source
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- hm_buffer (CultureMech:009705)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.268795Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3294 |
| 2026-01-28T19:48:08.449016Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:13.648159+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:08.276072Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009705 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.267195+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HM Buffer" to "hm_buffer" for consistent matching |
| 2026-05-16T09:03:37.222395+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:08.597644+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:37.678928+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ea3946239a582a1a0cdbb021b8996d049413cb4e54d225499e611c9694ac7727 |