Overview
- External ontology
- TOGO:M3294 — HM Buffer
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- TOGO M3294 imports NBRC Medium 1663 HM Buffer. NBRC 1663 prepares the buffer from 5 ml 0.5 M HEPES filled to 100 ml with UPW, adjusted to pH 7.4, autoclaved, then supplemented with 600 ul 0.5 M CaCl2 and 333 ul 0.6 M MgCl2. Volumes are normalized 10x from the 100 ml source recipe.
Composition
flowchart LR
CultureMech_009705["hm_buffer"]
ing0_UPW["UPW"]
CultureMech_009705 --> ing0_UPW
CHEBI_15377["CHEBI:15377"]
ing0_UPW -.-> CHEBI_15377
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
UPW
H2O 18.015 g/mol
|
1000.0 ML_PER_L |
CHEBI:15377
water
|
NBRC Medium 1663 fills the 0.5 M HEPES dilution to 100 ml with UPW, normalized here as q.s. to 1000.0 ml/L. |
Preparation
- Prepare filter-sterilized 0.5 M CaCl2 and 0.6 M MgCl2 stocks.
- Dilute 50.0 ml/L 0.5 M HEPES to 1000.0 ml/L with UPW.
- Adjust pH to 7.4.
- Sterilize the HEPES/UPW base by autoclaving.
- After autoclaving, add 6.0 ml/L 0.5 M CaCl2 stock and 3.33 ml/L 0.6 M MgCl2 stock.
Solutions
0.5 M HEPES
Dose: 50.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| HEPES | 0.5 MOLAR | NBRC Medium 1663 uses a 0.5 M HEPES stock. |
NBRC Medium 1663 dilutes 5 ml 0.5 M HEPES to 100 ml before pH adjustment and autoclaving.
0.5 M CaCl2 solution
Dose: 6.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2 | 0.5 MOLAR | NBRC Medium 1663 uses a 0.5 M CaCl2 stock. |
NBRC Medium 1663 adds 600 ul 0.5 M CaCl2 solution to the 100 ml autoclaved HEPES base.
0.6 M MgCl2 solution
Dose: 3.33 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgCl2 | 0.6 MOLAR | NBRC Medium 1663 uses a 0.6 M MgCl2 stock. |
NBRC Medium 1663 adds 333 ul 0.6 M MgCl2 solution to the 100 ml autoclaved HEPES base.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.268795Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3294 |
| 2026-01-28T19:48:08.449016Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:13.648159+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:08.276072Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009705 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.267195+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HM Buffer" to "hm_buffer" for consistent matching |
| 2026-05-16T09:03:37.222395+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:08.597644+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m3294_hm_buffer_score15.py | RESOLVED_TOGO_M3294_HM_BUFFER_SCORE15 | TOGO M3294 imports NBRC Medium 1663 HM Buffer. NBRC 1663 prepares the buffer from 5 ml 0.5 M HEPES filled to 100 ml with UPW, adjusted to pH 7.4, autoclaved, then supplemented with 600 ul 0.5 M CaCl2 and 333 ul 0.6 M MgCl2. Volumes are normalized 10x from the 100 ml source recipe. Converted the imported HEPES, UPW, CaCl2, and MgCl2 g/L artifacts to source volumes; expanded the salt stock solutions; added pH 7.4 and preparation steps; and grounded all disclosed chemical components. |