Overview
- External ontology
- TOGO:M406 — 15% MH Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M406 Original source: JCM - JCM_M408 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=408
Composition
flowchart LR
CultureMech_009790["15_mh_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009790 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009790 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009790 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009790 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009790 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_009790 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_NaHCO3["NaHCO3"]
CultureMech_009790 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Glucose["Glucose"]
CultureMech_009790 --> ing7_Glucose
CHEBI_17234["CHEBI:17234"]
ing7_Glucose -.-> CHEBI_17234
ing8_Agar["Agar"]
CultureMech_009790 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009790 --> ing9_Yeast_extract__BD_Difco_
ing10_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
CultureMech_009790 --> ing10_Proteose_peptone_No__3__BD_Difco_
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
14.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
121.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.54 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
10.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
3 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.09 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 9 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 10 | Yeast extract (BD-Difco) | 10 G_PER_L | Properties: Undefined component | |
| 11 | Proteose peptone No. 3 (BD-Difco) | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M406_15_MH_Medium (CultureMech:009790)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.998033Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M406 |
| 2026-01-28T19:48:25.185023Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:08.901269Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009790 |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.893188+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "15% MH Medium" to "15_mh_medium" for consistent matching |
| 2026-06-05T07:40:41.127057+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:30.319270+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-06T03:05:15.741346+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint cef1d1f593b5b3d896ec93b4546a9ecb293fb9bbf421aa4805a0734b1f3446be |