15_mh_medium (CultureMech:009790)

Overview

External ontology

TOGO:M406 — 15% MH Medium

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M406 Original source: JCM - JCM_M408 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=408

Composition

flowchart LR
    CultureMech_009790["15_mh_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009790 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009790 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_009790 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009790 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009790 --> ing4_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_6H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_009790 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NaHCO3["NaHCO3"]
    CultureMech_009790 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Glucose["Glucose"]
    CultureMech_009790 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_Agar["Agar"]
    CultureMech_009790 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009790 --> ing9_Yeast_extract__BD_Difco_
    ing10_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
    CultureMech_009790 --> ing10_Proteose_peptone_No__3__BD_Difco_

Ingredients (11)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2	MgSO4・7H2O 7H2O.Mg.O4S 246.473 g/mol	14.4 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
3	NaCl Cl.Na 58.443 g/mol	121.5 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4	CaCl2・2H2O Ca.2Cl.2H2O 147.014 g/mol	0.54 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
5	MgCl2・6H2O 2Cl.6H2O.Mg 203.301 g/mol	10.5 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
6	KCl Cl.K 74.551 g/mol	3 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
7	NaHCO3 CHO3.Na 84.006 g/mol	0.09 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
8	Glucose C6H12O6 180.156 g/mol	1 G_PER_L	CHEBI:17234 glucose	Role: Carbon source; Properties: Defined component, Organic compound, Simple component
9	Agar	20 G_PER_L	CHEBI:2509 agar	Role: Solidifying component
10	Yeast extract (BD-Difco)	10 G_PER_L		Properties: Undefined component
11	Proteose peptone No. 3 (BD-Difco)	5 G_PER_L		Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:54.998033Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M406
2026-01-28T19:48:25.185023Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:08.901269Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009790
2026-03-17T06:30:18.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:27.893188+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "15% MH Medium" to "15_mh_medium" for consistent matching
2026-06-05T07:40:41.127057+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:30.319270+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.