Overview
- External ontology
- TOGO:M458 — B Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M458 Original source: JCM - JCM_M458 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=458
Composition
flowchart LR
CultureMech_009845["b_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009845 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009845 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_009845 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Seawater["Seawater"]
CultureMech_009845 --> ing3_Seawater
ing4_Glucose["Glucose"]
CultureMech_009845 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
CultureMech_009845 --> ing5_Bacto_agar__BD_Difco_
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009845 --> ing6_Yeast_extract__BD_Difco_
ing7_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_009845 --> ing7_Bacto_peptone__BD_Difco_
ing8_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_009845 --> ing8_Casamino_acids__BD_Difco_
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
500 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 | Seawater | 500 G_PER_L | Role: Mineral source, Solvating media; Properties: Undefined component | |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 6 | Bacto agar (BD-Difco) | 15 G_PER_L | Role: Solidifying component | |
| 7 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 8 | Bacto peptone (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 9 | Casamino acids (BD-Difco) | 2 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M458_B_Medium (CultureMech:009845)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.096698Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M458 |
| 2026-01-28T19:48:08.372072Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:09.337428Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009845 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.309294+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "B Medium" to "b_medium" for consistent matching |
| 2026-06-05T07:40:41.543476+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:30.660399+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.682850+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:16.274976+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c88275d1f8bcd520bbd2bc4a9507d7b190e0492b8376e4b3f142c3057a23ee21 |