b_medium

CultureMech:009845 bacterial COMPLEX SOLID_AGAR
Original name: B Medium

Overview

External ontology
TOGO:M458 — B Medium
Applications
  • Microbial cultivation
pH range
{'min': 7.5, 'max': 7.8}
Notes
JCM Medium 458 B Medium lists, per liter, 500 ml distilled water, 500 ml seawater, 1 g glucose, 0.2 g KH2PO4, 0.05 g MgSO4 x 7H2O, 15 g Bacto agar from BD-Difco, and 2 g each of Bacto peptone, Casamino acids, and yeast extract from BD-Difco. Adjust pH to 7.5-7.8. The current JCM GRMD=458 page no longer exposes the recipe, but both TOGO M458 and MediaDive J458 preserve the JCM formulation.

Composition

flowchart LR
    CultureMech_009845["b_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009845 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_x_7H2O["MgSO4 x 7H2O"]
    CultureMech_009845 --> ing1_MgSO4_x_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7H2O -.-> CHEBI_31795
    ing2_KH2PO4["KH2PO4"]
    CultureMech_009845 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_Seawater["Seawater"]
    CultureMech_009845 --> ing3_Seawater
    ing4_Glucose["Glucose"]
    CultureMech_009845 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
    CultureMech_009845 --> ing5_Bacto_agar__BD_Difco_
    CHEBI_2509["CHEBI:2509"]
    ing5_Bacto_agar__BD_Difco_ -.-> CHEBI_2509
    ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009845 --> ing6_Yeast_extract__BD_Difco_
    ing7_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
    CultureMech_009845 --> ing7_Bacto_peptone__BD_Difco_
    ing8_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_009845 --> ing8_Casamino_acids__BD_Difco_

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
500.0 ML_PER_L CHEBI:15377
water
 TOGO M458 / JCM Medium 458 lists 500.0 ml/L Distilled water.
2 MgSO4 x 7H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 TOGO M458 / JCM Medium 458 lists 0.05 g/L MgSO4 x 7H2O.
3 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 TOGO M458 / JCM Medium 458 lists 0.2 g/L KH2PO4.
4 Seawater 500.0 ML_PER_L TOGO M458 / JCM Medium 458 lists 500 ml/L seawater as an undefined mineral source and solvent.
5 Glucose
C6H12O6 180.156 g/mol
1.0 G_PER_L CHEBI:17234
glucose
 TOGO M458 / JCM Medium 458 lists 1.0 g/L Glucose.
6 Bacto agar (BD-Difco) 15.0 G_PER_L CHEBI:2509
agar
 TOGO M458 / JCM Medium 458 lists 15.0 g/L Bacto agar from BD-Difco.
7 Yeast extract (BD-Difco) 2.0 G_PER_L TOGO M458 / JCM Medium 458 lists 2.0 g/L yeast extract from BD-Difco; this commercial mixture is retained as an opaque complex component.
8 Bacto peptone (BD-Difco) 2.0 G_PER_L TOGO M458 / JCM Medium 458 lists 2.0 g/L Bacto peptone from BD-Difco; this peptone is retained as an opaque complex component.
9 Casamino acids (BD-Difco) 2.0 G_PER_L TOGO M458 / JCM Medium 458 lists 2.0 g/L Casamino acids from BD-Difco; this amino-acid mixture is retained as an opaque complex component.

Preparation

  1. Adjust pH to 7.5-7.8.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.096698Z togo-import Imported from TOGO Medium Source: JCM, ID: M458
2026-01-28T19:48:08.372072Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:09.337428Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009845
2026-03-17T06:29:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.309294+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "B Medium" to "b_medium" for consistent matching
2026-06-05T07:40:41.543476+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:30.660399+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:50.682850+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00 repair_togo_m458_score15.py RESOLVED_TOGO_M458_SCORE15 JCM Medium 458 B Medium lists, per liter, 500 ml distilled water, 500 ml seawater, 1 g glucose, 0.2 g KH2PO4, 0.05 g MgSO4 x 7H2O, 15 g Bacto agar from BD-Difco, and 2 g each of Bacto peptone, Casamino acids, and yeast extract from BD-Difco. Adjust pH to 7.5-7.8. The current JCM GRMD=458 page no longer exposes the recipe, but both TOGO M458 and MediaDive J458 preserve the JCM formulation.