bacillus_filiformis_medium

CultureMech:009925 bacterial COMPLEX LIQUID
Original name: Bacillus Filiformis Medium

Overview

External ontology
TOGO:M533 — Bacillus Filiformis Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M533 Original source: JCM - JCM_M531 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=531

Composition

flowchart LR
    CultureMech_009925["bacillus_filiformis_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009925 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_009925 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_KCl["KCl"]
    CultureMech_009925 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_Na2CO3["Na2CO3"]
    CultureMech_009925 --> ing3_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing3_Na2CO3 -.-> CHEBI_29377
    ing4_Sodium_citrate["Sodium citrate"]
    CultureMech_009925 --> ing4_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing4_Sodium_citrate -.-> CHEBI_53258
    ing5_MgSO4_H2O["MgSO4・H2O"]
    CultureMech_009925 --> ing5_MgSO4_H2O
    CHEBI_32599["CHEBI:32599"]
    ing5_MgSO4_H2O -.-> CHEBI_32599
    ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009925 --> ing6_Yeast_extract__BD_Difco_
    ing7_MnCl2_4H2O["MnCl2・4H2O"]
    CultureMech_009925 --> ing7_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_4H2O -.-> CHEBI_86368
    ing8_FeSO4_H2O["FeSO4・H2O"]
    CultureMech_009925 --> ing8_FeSO4_H2O
    CHEBI_75834["CHEBI:75834"]
    ing8_FeSO4_H2O -.-> CHEBI_75834

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
3.0 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 3 duplicates: 1.0, 1.0, 1.0]
2 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
3 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 Na2CO3
CO3.2Na 105.988 g/mol
3 G_PER_L CHEBI:29377
sodium carbonate
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5 Sodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
Role: Buffer; Properties: Simple component, Organic compound, Defined component
6 MgSO4・H2O
Mg.O4S 120.368 g/mol
1 G_PER_L CHEBI:32599
magnesium sulfate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
7 Yeast extract (BD-Difco) 10 G_PER_L Properties: Undefined component
8 MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
0.36 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
9 FeSO4・H2O
Fe.H2O.O4S 169.923 g/mol
50 G_PER_L CHEBI:75834
iron(2+) sulfate monohydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component

Solutions

MnCl2 solution (see below)

Dose: 1 G_PER_L

FeSO4 solution (see below)

Dose: 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.243020Z togo-import Imported from TOGO Medium Source: JCM, ID: M533
2026-01-28T19:48:22.932334Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:38.128404+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T03:09:07.890344Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:09.998584Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009925
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.939635+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Bacillus Filiformis Medium" to "bacillus_filiformis_medium" for consistent matching
2026-05-16T09:03:18.827352+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:42.258231+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:50.902282+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:16.997772+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint b5fa3a39a1e19a7286557c4cd3dd5f2a732fb45121dcbb3b75fb48dfbe7976c2