Overview
- External ontology
- TOGO:M533 — Bacillus Filiformis Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M533 Original source: JCM - JCM_M531 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=531
Composition
flowchart LR
CultureMech_009925["bacillus_filiformis_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009925 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009925 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_KCl["KCl"]
CultureMech_009925 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Na2CO3["Na2CO3"]
CultureMech_009925 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_Sodium_citrate["Sodium citrate"]
CultureMech_009925 --> ing4_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing4_Sodium_citrate -.-> CHEBI_53258
ing5_MgSO4_H2O["MgSO4・H2O"]
CultureMech_009925 --> ing5_MgSO4_H2O
CHEBI_32599["CHEBI:32599"]
ing5_MgSO4_H2O -.-> CHEBI_32599
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009925 --> ing6_Yeast_extract__BD_Difco_
ing7_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_009925 --> ing7_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_4H2O -.-> CHEBI_86368
ing8_FeSO4_H2O["FeSO4・H2O"]
CultureMech_009925 --> ing8_FeSO4_H2O
CHEBI_75834["CHEBI:75834"]
ing8_FeSO4_H2O -.-> CHEBI_75834
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
100 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 3 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
3 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 6 |
MgSO4・H2O
Mg.O4S 120.368 g/mol
|
1 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 | Yeast extract (BD-Difco) | 10 G_PER_L | Properties: Undefined component | |
| 8 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.36 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 9 |
FeSO4・H2O
Fe.H2O.O4S 169.923 g/mol
|
50 G_PER_L |
CHEBI:75834
iron(2+) sulfate monohydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
Solutions
MnCl2 solution (see below)
Dose: 1 G_PER_L
FeSO4 solution (see below)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.243020Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M533 |
| 2026-01-28T19:48:22.932334Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:38.128404+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:07.890344Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:09.998584Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009925 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.939635+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Bacillus Filiformis Medium" to "bacillus_filiformis_medium" for consistent matching |
| 2026-05-16T09:03:18.827352+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:42.258231+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.902282+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:50.361288+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |