Overview
- External ontology
- TOGO:M549 — Desulfotomaculum PBE Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M549 Original source: JCM - JCM_M547 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=547
Composition
flowchart LR
CultureMech_009942["desulfotomaculum_pbe_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009942 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009942 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Na2SO4["Na2SO4"]
CultureMech_009942 --> ing2_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing2_Na2SO4 -.-> CHEBI_32149
ing3_CaCl2["CaCl2"]
CultureMech_009942 --> ing3_CaCl2
CHEBI_3312["CHEBI:3312"]
ing3_CaCl2 -.-> CHEBI_3312
ing4_Beef_extract["Beef extract"]
CultureMech_009942 --> ing4_Beef_extract
ing5_Peptone["Peptone"]
CultureMech_009942 --> ing5_Peptone
ing6_N2["N2"]
CultureMech_009942 --> ing6_N2
CHEBI_17997["CHEBI:17997"]
ing6_N2 -.-> CHEBI_17997
ing7_5__Sodium_lactate__autoclaved_["5% Sodium lactate (autoclaved)"]
CultureMech_009942 --> ing7_5__Sodium_lactate__autoclaved_
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.5 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 | Beef extract | 1 G_PER_L | Properties: Undefined component | |
| 6 | Peptone | 2 G_PER_L | ||
| 7 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 8 | 5% Sodium lactate (autoclaved) | 70 G_PER_L | Role: Carbon source; Properties: Defined component, Organic compound, Solution |
Solutions
4% Fe(NH4)2(SO4)2・6H2O solution (filter--sterilized)
Dose: 10 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
5% K2HPO4 solution (autoclaved)
Dose: 10 G_PER_L
Role: Buffer; Properties: Solution, Inorganic compound, Defined component
1% Sodium ascorbate solution (filter--sterilized)
Dose: 10 G_PER_L
Properties: Defined component, Organic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M549_Desulfotomaculum_PBE_Medium (CultureMech:009942)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.277555Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M549 |
| 2026-01-28T19:48:32.839026Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:54.341014+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:20:10.154703Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009942 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.085983+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Desulfotomaculum PBE Medium" to "desulfotomaculum_pbe_medium" for consistent matching |
| 2026-05-16T09:03:18.966397+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:42.409520+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:31.352816+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.955534+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:17.180007+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8088d97a807fc02b3cc0fa1db35e185b48ceed17bff4e579925cd9a17c378e58 |