desulfotomaculum_pbe_medium (CultureMech:009942)

Overview

External ontology

TOGO:M549 — Desulfotomaculum PBE Medium

Applications

Microbial cultivation

Notes

JCM Medium 547 Desulfotomaculum PBE Medium prepares Solution A from 2 g peptone, 1 g beef extract, 2 g MgSO4 x 7H2O, 1.5 g Na2SO4, 0.1 g CaCl2, and 900 ml distilled water, then autoclaves it under an N2 gas atmosphere. To complete the medium, 10 ml of autoclaved 5% K2HPO4, 70 ml of autoclaved 5% sodium lactate, 10 ml of filter-sterilized 4% Fe(NH4)2(SO4)2 x 6H2O, and 10 ml of filter-sterilized 1% sodium ascorbate are added aseptically and anaerobically per liter. JCM, TOGO, and MediaDive do not report a final pH.

Composition

flowchart LR
    CultureMech_009942["desulfotomaculum_pbe_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_009942 --> ing0_Peptone
    ing1_Beef_extract["Beef extract"]
    CultureMech_009942 --> ing1_Beef_extract
    ing2_MgSO4_x_7H2O["MgSO4 x 7H2O"]
    CultureMech_009942 --> ing2_MgSO4_x_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7H2O -.-> CHEBI_31795
    ing3_Na2SO4["Na2SO4"]
    CultureMech_009942 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_CaCl2["CaCl2"]
    CultureMech_009942 --> ing4_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing4_CaCl2 -.-> CHEBI_3312
    ing5_Distilled_water["Distilled water"]
    CultureMech_009942 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377
    ing6_N2["N2"]
    CultureMech_009942 --> ing6_N2
    CHEBI_17997["CHEBI:17997"]
    ing6_N2 -.-> CHEBI_17997

Ingredients (7)

#	Ingredient	Concentration	Ontology	Notes
1	Peptone	2.0 G_PER_L	MICRO:0000178 (source link unavailable) Peptone	TOGO M549 / JCM Medium 547 lists 2.0 g/L peptone.
2	Beef extract	1.0 G_PER_L	FOODON:03302088 Beef extract	TOGO M549 / JCM Medium 547 lists 1.0 g/L beef extract.
3	MgSO4 x 7H2O 7H2O.Mg.O4S 246.473 g/mol	2.0 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	TOGO M549 / JCM Medium 547 lists 2.0 g/L MgSO4 x 7H2O.
4	Na2SO4 2Na.O4S 142.043 g/mol	1.5 G_PER_L	CHEBI:32149 sodium sulfate	TOGO M549 / JCM Medium 547 lists 1.5 g/L Na2SO4.
5	CaCl2 Ca.2Cl 110.984 g/mol	0.1 G_PER_L	CHEBI:3312 calcium dichloride	TOGO M549 / JCM Medium 547 lists 0.1 g/L CaCl2.
6	Distilled water H2O 18.015 g/mol	900.0 ML_PER_L	CHEBI:15377 water	TOGO M549 / JCM Medium 547 lists 900.0 ml/L Distilled water.
7	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	TOGO M549 / JCM Medium 547 uses N2 as the gas atmosphere for autoclaving.

Preparation

Mix Solution A components and autoclave under an N2 gas atmosphere.
Aseptically and anaerobically add 10 ml/L autoclaved 5% K2HPO4 solution, 70 ml/L autoclaved 5% sodium lactate solution, 10 ml/L filter-sterilized 4% Fe(NH4)2(SO4)2 x 6H2O solution, and 10 ml/L filter-sterilized 1% sodium ascorbate solution.

Solutions

5% K2HPO4 solution

Dose: 10.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
K2HPO4	5.0 PERCENT_W_V	TOGO M549 / JCM Medium 547 specifies 5% K2HPO4 solution as 5.0% w/v.

TOGO M549 / JCM Medium 547 adds 10.0 ml/L 5% K2HPO4 solution.

5% Sodium lactate solution

Dose: 70.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Sodium lactate	5.0 PERCENT_W_V	TOGO M549 / JCM Medium 547 specifies 5% Sodium lactate solution as 5.0% w/v.

TOGO M549 / JCM Medium 547 adds 70.0 ml/L 5% Sodium lactate solution.

4% Fe(NH4)2(SO4)2 x 6H2O solution

Dose: 10.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Fe(NH4)2(SO4)2 x 6H2O	4.0 PERCENT_W_V	TOGO M549 / JCM Medium 547 specifies 4% Fe(NH4)2(SO4)2 x 6H2O solution as 4.0% w/v.

TOGO M549 / JCM Medium 547 adds 10.0 ml/L 4% Fe(NH4)2(SO4)2 x 6H2O solution.

1% Sodium ascorbate solution

Dose: 10.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Sodium ascorbate	1.0 PERCENT_W_V	TOGO M549 / JCM Medium 547 specifies 1% Sodium ascorbate solution as 1.0% w/v.

TOGO M549 / JCM Medium 547 adds 10.0 ml/L 1% Sodium ascorbate solution.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:55.277555Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M549
2026-01-28T19:48:32.839026Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: N2
2026-03-09T08:04:54.341014+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 3 solution(s) from ingredients
2026-03-13T06:20:10.154703Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009942
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:34.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.085983+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Desulfotomaculum PBE Medium" to "desulfotomaculum_pbe_medium" for consistent matching
2026-05-16T09:03:18.966397+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:42.409520+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:31.352816+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:50.955534+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00	repair_togo_m549_score15.py	RESOLVED_TOGO_M549_SCORE15	JCM Medium 547 Desulfotomaculum PBE Medium prepares Solution A from 2 g peptone, 1 g beef extract, 2 g MgSO4 x 7H2O, 1.5 g Na2SO4, 0.1 g CaCl2, and 900 ml distilled water, then autoclaves it under an N2 gas atmosphere. To complete the medium, 10 ml of autoclaved 5% K2HPO4, 70 ml of autoclaved 5% sodium lactate, 10 ml of filter-sterilized 4% Fe(NH4)2(SO4)2 x 6H2O, and 10 ml of filter-sterilized 1% sodium ascorbate are added aseptically and anaerobically per liter. JCM, TOGO, and MediaDive do not report a final pH.