alkaline_lb_agar

CultureMech:009956 bacterial COMPLEX SOLID_AGAR
Original name: Alkaline LB Agar

Overview

External ontology
TOGO:M561 — Alkaline LB Agar
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M561 Original source: JCM - JCM_M557 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=557

Composition

flowchart LR
    CultureMech_009956["alkaline_lb_agar"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009956 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_009956 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Na2CO3["Na2CO3"]
    CultureMech_009956 --> ing2_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing2_Na2CO3 -.-> CHEBI_29377
    ing3_Agar["Agar"]
    CultureMech_009956 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009956 --> ing4_Yeast_extract__BD_Difco_
    ing5_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_009956 --> ing5_Tryptone__BD_Difco_

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
15 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 Na2CO3
CO3.2Na 105.988 g/mol
5 G_PER_L CHEBI:29377
sodium carbonate
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
4 Agar 15 G_PER_L CHEBI:2509
agar
Role: Solidifying component
5 Yeast extract (BD-Difco) 5 G_PER_L Properties: Undefined component
6 Tryptone (BD-Difco) 10 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.305287Z togo-import Imported from TOGO Medium Source: JCM, ID: M561
2026-01-28T19:48:29.621782Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:10.280232Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009956
2026-03-17T06:30:28.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.210579+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Alkaline LB Agar" to "alkaline_lb_agar" for consistent matching
2026-06-05T07:40:42.554011+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:51.011523+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:17.349209+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint fdd5427726ad9a3e00c5189b3a7ea3943ac36d03620aed50aca6f5874f84b257