Overview
- External ontology
- TOGO:M694 — Ruminococcus Albus Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M694 Original source: JCM - JCM_M675 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=675 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_010100["ruminococcus_albus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010100 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Resazurin["Resazurin"]
CultureMech_010100 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_Na2CO3["Na2CO3"]
CultureMech_010100 --> ing2_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing2_Na2CO3 -.-> CHEBI_29377
ing3_Cellobiose["Cellobiose"]
CultureMech_010100 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Glucose["Glucose"]
CultureMech_010100 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010100 --> ing5_Yeast_extract__BD_Difco_
ing6_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010100 --> ing6_Tryptone__BD_Difco_
ing7_L__cysteine_HCl_H2O["L--cysteine・HCl・H2O"]
CultureMech_010100 --> ing7_L__cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L__cysteine_HCl_H2O -.-> CHEBI_91248
ing8_fatty_acid_mixture["fatty acid mixture"]
CultureMech_010100 --> ing8_fatty_acid_mixture
ing9_CO2["CO2"]
CultureMech_010100 --> ing9_CO2
CHEBI_16526["CHEBI:16526"]
ing9_CO2 -.-> CHEBI_16526
ing10_N2["N2"]
CultureMech_010100 --> ing10_N2
CHEBI_17997["CHEBI:17997"]
ing10_N2 -.-> CHEBI_17997
ing11_K2HPO4["K2HPO4"]
CultureMech_010100 --> ing11_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing11_K2HPO4 -.-> CHEBI_131527
ing12_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010100 --> ing12_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_7H2O -.-> CHEBI_31795
ing13_NaCl["NaCl"]
CultureMech_010100 --> ing13_NaCl
CHEBI_26710["CHEBI:26710"]
ing13_NaCl -.-> CHEBI_26710
ing14_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010100 --> ing14_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_2H2O -.-> CHEBI_86158
ing15_KH2PO4["KH2PO4"]
CultureMech_010100 --> ing15_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing15_KH2PO4 -.-> CHEBI_63036
ing16__NH4_2SO4["(NH4)2SO4"]
CultureMech_010100 --> ing16__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing16__NH4_2SO4 -.-> CHEBI_62946
ing17_Isovaleric_acid["Isovaleric acid"]
CultureMech_010100 --> ing17_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing17_Isovaleric_acid -.-> CHEBI_28484
ing18_Isobutyric_acid["Isobutyric acid"]
CultureMech_010100 --> ing18_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing18_Isobutyric_acid -.-> CHEBI_16135
ing19_Tryptone["Tryptone"]
CultureMech_010100 --> ing19_Tryptone
ing20_Yeast_extract["Yeast extract"]
CultureMech_010100 --> ing20_Yeast_extract
ing21_Sodium_chloride["Sodium chloride"]
CultureMech_010100 --> ing21_Sodium_chloride
ing21_Sodium_chloride -.-> CHEBI_26710
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1190.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 920.0, 100.0, 100.0, 70.0] |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 3 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
2 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
3 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 7 | Tryptone (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 8 |
L--cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
500 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 9 | fatty acid mixture | 1 G_PER_L | ||
| 10 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 12 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
NaCl
Cl.Na 58.443 g/mol
|
1.2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.16 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
10 G_PER_L |
CHEBI:28484
isovaleric acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 19 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
10 G_PER_L |
CHEBI:16135
isobutyric acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 20 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 21 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 22 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Solutions
Mineral solution 1 (see below)
Dose: 40 G_PER_L
Mineral solution 2 (see below)
Dose: 40 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M694_Ruminococcus_Albus_Medium (CultureMech:010100)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.543954Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M694 |
| 2026-01-28T19:48:38.458206Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2; Added default concentration for ingredient: N2 |
| 2026-03-09T08:05:04.338557+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:36.297550Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:11.306210Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010100 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.235282+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ruminococcus Albus Medium" to "ruminococcus_albus_medium" for consistent matching |
| 2026-05-16T09:03:20.122129+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:43.632739+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.389689+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.357412+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:18.486958+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 11635e7bb87b50fa56aa3bebb40408e1455d8c2bf8bde208a830cb8c21006cbf |