Overview
- External ontology
- TOGO:M694 — Ruminococcus Albus Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M694 Original source: JCM - JCM_M675 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=675 Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. Preparation order is preserved as source text; provider paragraph grouping does not make all inputs simultaneous: Boil and cool under CO2. Under a gentle stream of CO2, add 4.0 g Na2CO3, 1.0 ml fatty acid mixture (see below) and 500 mg L--cysteine・HCl・H2O. Adjust pH to 7.0, distribute into appropriate vessels under N2 and autoclave. Original-source cross-check retains source-specific preparation stages and calcium hydrate labels. Similar names do not establish exact equivalence, percentage basis or a final-volume denominator. Complete source rows determine the reconstructed formulation. Archive the prior flattened nodes, unsupported LB additions and stock links in append-only history. Unrelated annotations are preserved, not recertified. Complete source rows determine the reconstructed formulation. Archive the prior flattened nodes, unsupported LB additions and stock links in append-only history. Unrelated annotations are preserved, not recertified.
Composition
flowchart LR
CultureMech_010100["ruminococcus_albus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010100 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Resazurin["Resazurin"]
CultureMech_010100 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_Na2CO3["Na2CO3"]
CultureMech_010100 --> ing2_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing2_Na2CO3 -.-> CHEBI_29377
ing3_Cellobiose["Cellobiose"]
CultureMech_010100 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Glucose["Glucose"]
CultureMech_010100 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010100 --> ing5_Yeast_extract__BD_Difco_
ing6_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010100 --> ing6_Tryptone__BD_Difco_
ing7_L__cysteine_HCl_H2O["L--cysteine・HCl・H2O"]
CultureMech_010100 --> ing7_L__cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L__cysteine_HCl_H2O -.-> CHEBI_91248
ing8_CO2["CO2"]
CultureMech_010100 --> ing8_CO2
CHEBI_16526["CHEBI:16526"]
ing8_CO2 -.-> CHEBI_16526
ing9_N2["N2"]
CultureMech_010100 --> ing9_N2
CHEBI_17997["CHEBI:17997"]
ing9_N2 -.-> CHEBI_17997
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
0.92 L |
CHEBI:15377
water
|
Superseded flattening history, not a current amount: Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 920.0, 100.0, 100.0, 70.0] Source input only; not final volume or percentage Source input: 920 ml at components[0].items[0]. |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 1 mg at components[0].items[1]. | |
| 3 |
Na2CO3
CO3.2Na 105.988 g/mol
|
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 4 g at components[0].items[2]. | |
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 2 g at components[0].items[3]. | |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 3 g at components[0].items[4]. | |
| 6 | Yeast extract (BD-Difco) | Properties: Undefined component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 2 g at components[0].items[5]. | ||
| 7 | Tryptone (BD-Difco) | Properties: Undefined component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 5 g at components[0].items[6]. | ||
| 8 |
L--cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound Keep source mass and preparation stage in evidenced notes, not g/L Source input: 500 mg at components[0].items[7]. | |
| 9 |
CO2
CO2 44.009 g/mol
|
CHEBI:16526
carbon dioxide
|
Properties: ガス No numeric source dose; never turn missing into zero Source input: None None at components[0].items[11]. | |
| 10 |
N2
N2 28.014 g/mol
|
CHEBI:17997
dinitrogen
|
Properties: ガス No numeric source dose; never turn missing into zero Source input: None None at components[0].items[12]. |
Solutions
Mineral solution 1 (see below)
Dose: 0.04 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 0.1 L | Superseded flattening history, not a current amount: Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 920.0, 100.0, 100.0, 70.0] Source input only; not final volume or percentage Source input: 100 ml at components[1].items[0]. |
| K2HPO4 | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 0.6 g at components[1].items[1]. |
Source input only; not final volume or percentage Source input: 40 ml at components[0].items[8].
Mineral solution 2 (see below)
Dose: 0.04 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 0.1 L | Superseded flattening history, not a current amount: Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 920.0, 100.0, 100.0, 70.0] Source input only; not final volume or percentage Source input: 100 ml at components[2].items[0]. |
| MgSO4・7H2O | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 0.25 g at components[2].items[1]. | |
| NaCl | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 1.2 g at components[2].items[2]. | |
| CaCl2・2H2O | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 0.16 g at components[2].items[3]. | |
| KH2PO4 | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 0.6 g at components[2].items[4]. | |
| (NH4)2SO4 | Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 2 g at components[2].items[5]. |
Source input only; not final volume or percentage Source input: 40 ml at components[0].items[9].
fatty acid mixture
Dose: 0.001 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 0.07 L | Superseded flattening history, not a current amount: Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 920.0, 100.0, 100.0, 70.0] Source input only; not final volume or percentage Source input: 70 ml at components[3].items[0]. |
| Isovaleric acid | 0.01 L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source input only; not final volume or percentage Source input: 10 ml at components[3].items[1]. |
| Isobutyric acid | 0.01 L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source input only; not final volume or percentage Source input: 10 ml at components[3].items[2]. |
| 2--Methylbutyric acid | 0.01 L | Source input only; not final volume or percentage Source input: 10 ml at components[3].items[3]. |
Source input only; not final volume or percentage Source input: 1 ml at components[0].items[10].
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.543954Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M694 |
| 2026-01-28T19:48:38.458206Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2; Added default concentration for ingredient: N2 |
| 2026-03-09T08:05:04.338557+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:36.297550Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:11.306210Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010100 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.235282+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ruminococcus Albus Medium" to "ruminococcus_albus_medium" for consistent matching |
| 2026-05-16T09:03:20.122129+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:43.632739+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.389689+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.357412+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-09T07:30:35.561516+00:00 | codex | RESTORED_RUMINOCOCCUS_ALBUS_SOURCE_FORMULATIONS_WITH_EVIDENCE | Restore exact-source medium436 formulation, preparation stages and input units. Preserve qualified prior rates, source-specific nulls and unresolved original bases. No growth association added. |