Overview
- External ontology
- TOGO:M6 — BL Agar (Glucose Blood Liver Agar)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M6 Original source: JCM - JCM_M13 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=13
Composition
flowchart LR
CultureMech_010107["bl_agar_glucose_blood_liver_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_010107 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Tween_80["Tween 80"]
CultureMech_010107 --> ing1_Tween_80
CHEBI_53426["CHEBI:53426"]
ing1_Tween_80 -.-> CHEBI_53426
ing2_Horse_blood["Horse blood"]
CultureMech_010107 --> ing2_Horse_blood
ing3_Glucose["Glucose"]
CultureMech_010107 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Soluble_starch["Soluble starch"]
CultureMech_010107 --> ing4_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing4_Soluble_starch -.-> CHEBI_28017
ing5_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
CultureMech_010107 --> ing5_Bacto_agar__BD_Difco_
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010107 --> ing6_Yeast_extract__BD_Difco_
ing7_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_010107 --> ing7_Trypticase_peptone__BD_BBL_
ing8_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
CultureMech_010107 --> ing8_Proteose_peptone_No__3__BD_Difco_
ing9_Phytone_peptone__BD_BBL_["Phytone peptone (BD-BBL)"]
CultureMech_010107 --> ing9_Phytone_peptone__BD_BBL_
ing10_Lab__Lemco_powder__Oxoid_["Lab--Lemco powder (Oxoid)"]
CultureMech_010107 --> ing10_Lab__Lemco_powder__Oxoid_
ing11_Liver_extract__see_below_["Liver extract (see below)"]
CultureMech_010107 --> ing11_Liver_extract__see_below_
ing12_water["water"]
CultureMech_010107 --> ing12_water
ing12_water -.-> CHEBI_15377
ing13_liver_powder["liver powder"]
CultureMech_010107 --> ing13_liver_powder
ing14_KH2PO4["KH2PO4"]
CultureMech_010107 --> ing14_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing14_KH2PO4 -.-> CHEBI_63036
ing15_K2HPO4["K2HPO4"]
CultureMech_010107 --> ing15_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing15_K2HPO4 -.-> CHEBI_131527
ing16_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010107 --> ing16_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing16_MgSO4_7H2O -.-> CHEBI_31795
ing17_NaCl["NaCl"]
CultureMech_010107 --> ing17_NaCl
CHEBI_26710["CHEBI:26710"]
ing17_NaCl -.-> CHEBI_26710
ing18_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010107 --> ing18_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4_7H2O -.-> CHEBI_75836
ing19_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_010107 --> ing19_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing19_MnSO4_xH2O -.-> CHEBI_86360
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1025.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 3 duplicates: 825.0, 100.0, 100.0] |
| 2 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Defined component, Organic compound, Simple component |
| 3 | Horse blood | 50 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 5 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 6 | Bacto agar (BD-Difco) | 15 G_PER_L | Role: Solidifying component | |
| 7 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 8 | Trypticase peptone (BD-BBL) | 5 G_PER_L | Properties: Undefined component | |
| 9 | Proteose peptone No. 3 (BD-Difco) | 10 G_PER_L | Properties: Undefined component | |
| 10 | Phytone peptone (BD-BBL) | 3 G_PER_L | Properties: Undefined component | |
| 11 | Lab--Lemco powder (Oxoid) | 3 G_PER_L | Properties: Undefined component | |
| 12 | Liver extract (see below) | 150 G_PER_L | ||
| 13 |
water
H2O 18.015 g/mol
|
170 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 14 | liver powder | 10 G_PER_L | Properties: Undefined component | |
| 15 |
KH2PO4
H2O4P.K 136.084 g/mol
|
10 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 16 |
K2HPO4
HO4P.2K 174.174 g/mol
|
10 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 17 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 18 |
NaCl
Cl.Na 58.443 g/mol
|
0.2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 19 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 20 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.2 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
5% L--Cysteine・HCl・H2O solution
Dose: 10 G_PER_L
Properties: Defined component, Organic compound, Solution
Solution A (see below)
Dose: 10 G_PER_L
Solution B (see below)
Dose: 5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M6_BL_Agar_Glucose_Blood_Liver_Agar (CultureMech:010107)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.377853Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M6 |
| 2026-01-28T19:48:15.806046Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:26.074636+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:54.619117Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:11.370204Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010107 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.299461+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BL Agar (Glucose Blood Liver Agar)" to "bl_agar_glucose_blood_liver_agar" for consistent matching |
| 2026-05-16T09:03:20.195179+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:43.706171+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.448983+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.387492+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:18.581654+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 982cac87e87f2dd34911da3df208809961602eec934ff45ea481c30aa483c4dc |