bl_agar_glucose_blood_liver_agar

Overview

External ontology

TOGO:M6 — BL Agar (Glucose Blood Liver Agar)

Applications

• Microbial cultivation

pH

7.2

Notes

JCM Medium 13 BL Agar lists Lab-Lemco powder, three peptone products, yeast extract, liver extract, glucose, soluble starch, Solution A, Solution B, Tween 80, agar, 5% L-Cysteine HCl x H2O solution, horse blood, and distilled water; the formula is adjusted to pH 7.2 and horse blood is added aseptically after autoclaving and cooling.

Composition

flowchart LR
    CultureMech_010107["bl_agar_glucose_blood_liver_agar"]
    ing0_Lab_Lemco_powder__Oxoid_["Lab-Lemco powder (Oxoid)"]
    CultureMech_010107 --> ing0_Lab_Lemco_powder__Oxoid_
    ing1_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
    CultureMech_010107 --> ing1_Proteose_peptone_No__3__BD_Difco_
    ing2_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_010107 --> ing2_Trypticase_peptone__BD_BBL_
    ing3_Phytone_peptone__BD_BBL_["Phytone peptone (BD-BBL)"]
    CultureMech_010107 --> ing3_Phytone_peptone__BD_BBL_
    ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010107 --> ing4_Yeast_extract__BD_Difco_
    ing5_Glucose["Glucose"]
    CultureMech_010107 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Soluble_starch["Soluble starch"]
    CultureMech_010107 --> ing6_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing6_Soluble_starch -.-> CHEBI_28017
    ing7_Tween_80["Tween 80"]
    CultureMech_010107 --> ing7_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing7_Tween_80 -.-> CHEBI_53426
    ing8_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
    CultureMech_010107 --> ing8_Bacto_agar__BD_Difco_
    CHEBI_2509["CHEBI:2509"]
    ing8_Bacto_agar__BD_Difco_ -.-> CHEBI_2509
    ing9_Horse_blood["Horse blood"]
    CultureMech_010107 --> ing9_Horse_blood
    ing10_Distilled_water["Distilled water"]
    CultureMech_010107 --> ing10_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing10_Distilled_water -.-> CHEBI_15377

Ingredients (11)

Preparation

1. Prepare Liver extract by putting 10.0 g liver powder in 170.0 ml water, holding at 50-60 C for 1 hr, boiling for 5 min, adjusting pH to 7.2, and filtering.
2. Combine the base ingredients, 150.0 ml/L Liver extract, 10.0 ml/L Solution A, 5.0 ml/L Solution B, 10.0 ml/L 5% L-Cysteine HCl x H2O solution, and 825.0 ml/L distilled water; omit horse blood before autoclaving.
3. Adjust pH to 7.2 before autoclaving.
4. Autoclave at 121 C for 15 min.
5. Cool to 50 C and aseptically add 50.0 ml/L horse blood.

Solutions

Target organisms

No target organisms are recorded.

Provenance