Overview
- External ontology
- TOGO:M765 — MVL Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M765 Original source: JCM - JCM_M740 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=740
Composition
flowchart LR
CultureMech_010172["mvl_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010172 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Glucose["Glucose"]
CultureMech_010172 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Agar["Agar"]
CultureMech_010172 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010172 --> ing3_Yeast_extract__BD_Difco_
ing4_Beef_extract["Beef extract"]
CultureMech_010172 --> ing4_Beef_extract
ing5_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_010172 --> ing5_Trypticase_peptone__BD_BBL_
ing6_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010172 --> ing6_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_7H2O -.-> CHEBI_31795
ing7_NaCl["NaCl"]
CultureMech_010172 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
ing8_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010172 --> ing8_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_2H2O -.-> CHEBI_86158
ing9_K2HPO4["K2HPO4"]
CultureMech_010172 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10__NH4_2SO4["(NH4)2SO4"]
CultureMech_010172 --> ing10__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing10__NH4_2SO4 -.-> CHEBI_62946
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
101.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 1.0, 100.0] |
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 4 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 5 | Beef extract | 2 G_PER_L | Properties: Undefined component | |
| 6 | Trypticase peptone (BD-BBL) | 10 G_PER_L | Properties: Undefined component | |
| 7 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.12 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
1.2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 9 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.12 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 11 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
Solutions
8.0% Na2CO3 solution
Dose: 50 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
0.1% Resazurin solution
Dose: 1 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
0.07% Hemin solution
Dose: 10 G_PER_L
Role: Growth factor; Properties: Organic compound, Solution, Defined component
3.0% L--Cysteine・HCl・H2O solution
Dose: 10 G_PER_L
Properties: Defined component, Organic compound, Solution
Mineral solution 1 (see below)
Dose: 75 G_PER_L
Mineral solution 2 (see below)
Dose: 75 G_PER_L
KH2PO4 solution
Dose: variable VARIABLE
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M765_MVL_Medium (CultureMech:010172)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.667736Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M765 |
| 2026-01-28T19:48:27.512332Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KH2PO4 solution |
| 2026-03-09T08:04:45.546234+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:09:16.014059Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:11.838994Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010172 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.773672+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MVL Medium" to "mvl_medium" for consistent matching |
| 2026-05-16T09:03:20.664161+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.210413+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.853066+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.561424+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:19.073377+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d28c0d24a4e73e23659601558881c2557f140dd6e79c6208f8f5deb3fc9e8a97 |