mvl_medium

CultureMech:010172 bacterial COMPLEX SOLID_AGAR
Original name: MVL Medium

Overview

External ontology
TOGO:M765 — MVL Medium
Applications
  • Microbial cultivation
Notes
JCM Medium 740, MediaDive J740, and TOGO M765 describe MVL Medium as a 1.221 L main solution containing 1.0 L distilled water, 75.0 ml each of Mineral solution 1 and Mineral solution 2, 1.0 ml of 0.1% resazurin, 50.0 ml of 8% Na2CO3, 10.0 ml each of 0.07% hemin and 3% L-Cysteine HCl x H2O stocks, Trypticase peptone, yeast extract, beef extract, glucose, and agar.

Composition

flowchart LR
    CultureMech_010172["mvl_medium"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_010172 --> ing0_Trypticase_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_010172 --> ing1_Yeast_extract
    ing2_Beef_extract["Beef extract"]
    CultureMech_010172 --> ing2_Beef_extract
    ing3_Agar["Agar"]
    CultureMech_010172 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Glucose["Glucose"]
    CultureMech_010172 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Distilled_water["Distilled water"]
    CultureMech_010172 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Trypticase peptone 8.19001 G_PER_L TOGO M765 / JCM Medium 740 lists 8.19001 g/L Trypticase peptone.
2 Yeast extract 4.095 G_PER_L FOODON:03315426
yeast extract
 TOGO M765 / JCM Medium 740 lists 4.095 g/L Yeast extract.
3 Beef extract 1.638 G_PER_L TOGO M765 / JCM Medium 740 lists 1.638 g/L Beef extract.
4 Agar 12.285 G_PER_L CHEBI:2509
agar
 TOGO M765 / JCM Medium 740 lists 12.285 g/L Agar.
5 Glucose
C6H12O6 180.156 g/mol
1.638 G_PER_L CHEBI:17234
glucose
 TOGO M765 / JCM Medium 740 lists 1.638 g/L Glucose.
6 Distilled water
H2O 18.015 g/mol
819.001 ML_PER_L CHEBI:15377
water
 TOGO M765 / JCM Medium 740 contributes 819.001 ml/L distilled water.

Preparation

  1. Prepare the main MVL Medium from 819.001 ml/L distilled water, 61.4251 ml/L each of Mineral solution 1 and Mineral solution 2, 0.819001 ml/L 0.1% Resazurin solution, 40.95 ml/L 8% Na2CO3 solution, 8.19001 ml/L each of 0.07% Hemin solution and 3% L-Cysteine HCl x H2O solution, Trypticase peptone, yeast extract, beef extract, glucose, and agar.
  2. Autoclave at 121 C for 15 min unless otherwise stated.

Solutions

Mineral solution 1

Dose: 61.4251 ML_PER_L

Stock composition
IngredientConcentrationNotes
KH2PO4 0.6 PERCENT_W_V TOGO M765 / JCM Medium 740 describes Mineral solution 1 as 0.6% KH2PO4.
TOGO M765 / JCM Medium 740 adds 61.4251 ml/L Mineral solution 1.

Mineral solution 2

Dose: 61.4251 ML_PER_L

Stock composition
IngredientConcentrationNotes
K2HPO4 6.0 G_PER_L TOGO M765 / JCM Medium 740 lists 6.0 g/L K2HPO4.
(NH4)2SO4 12.0 G_PER_L TOGO M765 / JCM Medium 740 lists 12.0 g/L (NH4)2SO4.
NaCl 12.0 G_PER_L TOGO M765 / JCM Medium 740 lists 12.0 g/L NaCl.
MgSO4 x 7 H2O 1.2 G_PER_L TOGO M765 / JCM Medium 740 lists 1.2 g/L MgSO4 x 7 H2O.
CaCl2 x 2 H2O 1.2 G_PER_L TOGO M765 / JCM Medium 740 lists 1.2 g/L CaCl2 x 2 H2O.
Distilled water 1000.0 ML_PER_L TOGO M765 / JCM Medium 740 makes Mineral solution 2 up with 100.0 ml water.
TOGO M765 / JCM Medium 740 adds 61.4251 ml/L Mineral solution 2.

0.1% Resazurin solution

Dose: 0.819001 ML_PER_L

Stock composition
IngredientConcentrationNotes
Resazurin 0.1 PERCENT_W_V TOGO M765 / JCM Medium 740 specifies 0.1% Resazurin solution as 0.1% w/v.
TOGO M765 / JCM Medium 740 adds 0.819001 ml/L 0.1% Resazurin solution.

0.07% Hemin solution

Dose: 8.19001 ML_PER_L

Stock composition
IngredientConcentrationNotes
Hemin 0.07 PERCENT_W_V TOGO M765 / JCM Medium 740 specifies 0.07% Hemin solution as 0.07% w/v.
TOGO M765 / JCM Medium 740 adds 8.19001 ml/L 0.07% Hemin solution.

8% Na2CO3 solution

Dose: 40.95 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2CO3 8.0 PERCENT_W_V TOGO M765 / JCM Medium 740 specifies 8% Na2CO3 solution as 8.0% w/v.
TOGO M765 / JCM Medium 740 adds 40.95 ml/L 8% Na2CO3 solution.

3% L-Cysteine HCl x H2O solution

Dose: 8.19001 ML_PER_L

Stock composition
IngredientConcentrationNotes
L-Cysteine HCl x H2O 3.0 PERCENT_W_V TOGO M765 / JCM Medium 740 specifies 3% L-Cysteine HCl x H2O solution as 3.0% w/v.
TOGO M765 / JCM Medium 740 adds 8.19001 ml/L 3% L-Cysteine HCl x H2O solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.667736Z togo-import Imported from TOGO Medium Source: JCM, ID: M765
2026-01-28T19:48:27.512332Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: KH2PO4 solution
2026-03-09T08:04:45.546234+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 7 solution(s) from ingredients
2026-03-13T03:09:16.014059Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:11.838994Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010172
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:30.773672+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MVL Medium" to "mvl_medium" for consistent matching
2026-05-16T09:03:20.664161+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:44.210413+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:32.853066+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:51.561424+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00 repair_togo_m765_mvl_score15.py RESOLVED_TOGO_M765_MVL_MEDIUM Moved Mineral solution 1, Mineral solution 2, 0.1% Resazurin, 0.07% Hemin, 8% Na2CO3, 3% L-Cysteine HCl x H2O, and KH2PO4 from empty solution stubs into structured solution entries, corrected main and stock water units, and linked the MediaDive J740 source duplicate.