Overview
- External ontology
- TOGO:M803 — Mineral Medium For Hydrogenophilus Islandicum
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M803 Original source: JCM - JCM_M774 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=774
Composition
flowchart LR
CultureMech_010215["mineral_medium_for_hydrogenophilus_islandicum"]
ing0_Sol__1["Sol. 1"]
CultureMech_010215 --> ing0_Sol__1
ing1_Sol__2["Sol. 2"]
CultureMech_010215 --> ing1_Sol__2
ing2_Distilled_water["Distilled water"]
CultureMech_010215 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010215 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_010215 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_010215 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_NaHCO3["NaHCO3"]
CultureMech_010215 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Na2HPO4_6H2O["Na2HPO4・6H2O"]
CultureMech_010215 --> ing7_Na2HPO4_6H2O
CHEBI_34683["CHEBI:34683"]
ing7_Na2HPO4_6H2O -.-> CHEBI_34683
ing8_0_1__Fe_NH4__citrate["0.1% Fe(NH4) citrate"]
CultureMech_010215 --> ing8_0_1__Fe_NH4__citrate
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010215 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_Hydrogen_gas["Hydrogen gas"]
CultureMech_010215 --> ing10_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing10_Hydrogen_gas -.-> CHEBI_18276
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sol. 1 | 17.6 G_PER_L | ||
| 2 | Sol. 2 | 2.4 G_PER_L | ||
| 3 |
Distilled water
H2O 18.015 g/mol
|
880 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 8 |
Na2HPO4・6H2O
HO4P.2Na 141.958 g/mol
|
2.9 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 9 | 0.1% Fe(NH4) citrate | 0.4 G_PER_L | Role: Nitrogen source; Properties: Defined component, Organic compound, Solution | |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Solutions
SL-6 trace element solution (see Medium [M158])
Dose: 5 G_PER_L
Cross-reference to Medium M158
0.5% MgSO4・7H2O solution
Dose: 2 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M803_Mineral_Medium_For_Hydrogenophilus_Islandicum (CultureMech:010215)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.746576Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M803 |
| 2026-01-28T19:48:07.499668Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:12.069426+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:12.173245Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010215 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.114672+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mineral Medium For Hydrogenophilus Islandicum" to "mineral_medium_for_hydrogenophilus_islandicum" for consistent matching |
| 2026-05-16T09:03:21.014574+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.599836+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.689414+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:19.462960+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 79a54d9ec581f773838dd5dd07afb43b84b08b151216ad9b270405cd59c56f93 |