Overview
- External ontology
- TOGO:M803 — Mineral Medium For Hydrogenophilus Islandicum
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- TOGO M803 mirrors JCM Medium 774: Mineral Medium for Hydrogenophilus islandicum combines the basal salts, SL-6 trace elements, autoclaved magnesium sulfate and ferric ammonium citrate stocks, and H2-CO2 pressurization.
Composition
flowchart LR
CultureMech_010215["mineral_medium_for_hydrogenophilus_islandicum"]
ing0_KH2PO4["KH2PO4"]
CultureMech_010215 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4_x_6_H2O["Na2HPO4 x 6 H2O"]
CultureMech_010215 --> ing1_Na2HPO4_x_6_H2O
ing2_NH4Cl["NH4Cl"]
CultureMech_010215 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaHCO3["NaHCO3"]
CultureMech_010215 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_010215 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Distilled_water["Distilled water"]
CultureMech_010215 --> ing5_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing5_Distilled_water -.-> CHEBI_15377
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.30 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
JCM Medium 774 prints this component. |
| 2 | Na2HPO4 x 6 H2O | 2.9 G_PER_L | JCM Medium 774 prints this component. | |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.0 G_PER_L |
CHEBI:31206
ammonium chloride
|
JCM Medium 774 prints this component. |
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
JCM Medium 774 prints this component. |
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
JCM Medium 774 prints this component. |
| 6 |
Distilled water
H2O 18.015 g/mol
|
880.0 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 774 lists 880.0 ml_per_l distilled water. |
Preparation
- Mix Mineral Medium for Hydrogenophilus islandicum components thoroughly and adjust pH to 6.8.
- Autoclave the basal medium.
- After cooling, add autoclaved 0.5% MgSO4 x 7 H2O and 0.1% Fe(NH4) citrate solutions.
- Aseptically distribute the medium into culture vessels, seal with butyl rubber stoppers, and pressurize to 100 kPa with H2-CO2 (4:1, v/v).
Solutions
SL-6 trace element solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| ZnSO4 x 7 H2O | 0.1 G_PER_L | JCM Medium 167 prints this component in its solution subrecipe. |
| MnCl2 x 4 H2O | 0.03 G_PER_L | JCM Medium 167 prints this component in its solution subrecipe. |
| H3BO3 | 0.3 G_PER_L | JCM Medium 167 prints this component in its solution subrecipe. |
| CoCl2 x 6 H2O | 0.2 G_PER_L | JCM Medium 167 prints this component in its solution subrecipe. |
| CuCl2 x 2 H2O | 0.01 G_PER_L | JCM Medium 167 prints this component in its solution subrecipe. |
| NiCl2 x 6 H2O | 0.02 G_PER_L | JCM Medium 167 prints this component in its solution subrecipe. |
| Na2MoO4 x 2 H2O | 0.03 G_PER_L | JCM Medium 167 prints this component in its solution subrecipe. |
| Distilled water | 1.0 L | JCM Medium 167 lists 1.0 l distilled water. |
JCM Medium 774 adds 5.0 mL/L SL-6 trace element solution from JCM Medium 167.
0.5% MgSO4 x 7 H2O solution
Dose: 100.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4 x 7 H2O | 0.5 PERCENT_W_V | JCM Medium 774 prints this component in its solution subrecipe. |
JCM Medium 774 adds 100.0 mL/L 0.5% MgSO4 x 7 H2O solution.
0.1% Fe(NH4) citrate solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Fe(NH4) citrate | 0.1 PERCENT_W_V | JCM Medium 774 prints this component in its solution subrecipe. |
JCM Medium 774 adds 20.0 mL/L 0.1% Fe(NH4) citrate solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.746576Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M803 |
| 2026-01-28T19:48:07.499668Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:12.069426+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:12.173245Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010215 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.114672+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mineral Medium For Hydrogenophilus Islandicum" to "mineral_medium_for_hydrogenophilus_islandicum" for consistent matching |
| 2026-05-16T09:03:21.014574+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.599836+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.689414+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-06T00:00:00-07:00 | repair_jcm_more_wrappers_score40.py | RESOLVED_JCM_MORE_SCORE40_GRAPH | TOGO M803 mirrors JCM Medium 774: Mineral Medium for Hydrogenophilus islandicum combines the basal salts, SL-6 trace elements, autoclaved magnesium sulfate and ferric ammonium citrate stocks, and H2-CO2 pressurization. |
| 2026-09-06T00:00:00-07:00 | repair_jcm_more_wrappers_score40.py | LINKED_JCM_MORE_SCORE40_CHILDREN | JCM Medium 775 adjusts JCM Medium 774 to pH 4.0. |