Overview
- External ontology
- TOGO:M809 — Modified Haloanaerobium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M809 Original source: JCM - JCM_M779 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=779
Composition
flowchart LR
CultureMech_010221["modified_haloanaerobium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010221 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010221 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_010221 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_010221 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KCl["KCl"]
CultureMech_010221 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_010221 --> ing5_Trypticase_peptone__BD_BBL_
ing6_NaOH["NaOH"]
CultureMech_010221 --> ing6_NaOH
CHEBI_32145["CHEBI:32145"]
ing6_NaOH -.-> CHEBI_32145
ing7_N2["N2"]
CultureMech_010221 --> ing7_N2
CHEBI_17997["CHEBI:17997"]
ing7_N2 -.-> CHEBI_17997
ing8_Sodium_thioglycolate["Sodium thioglycolate"]
CultureMech_010221 --> ing8_Sodium_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing8_Sodium_thioglycolate -.-> CHEBI_86481
ing9_Sodium_ascorbate["Sodium ascorbate"]
CultureMech_010221 --> ing9_Sodium_ascorbate
CHEBI_113451["CHEBI:113451"]
ing9_Sodium_ascorbate -.-> CHEBI_113451
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
26.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 1.0, 25.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
8.8 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Yeast extract | 10 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
75 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 6 | Trypticase peptone (BD-BBL) | 10 G_PER_L | Properties: Undefined component | |
| 7 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 8 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 9 |
Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.5 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Properties: Simple component, Defined component, Organic compound |
| 10 |
Sodium ascorbate
C6H7O6.Na 198.106 g/mol
|
0.5 G_PER_L |
CHEBI:113451
sodium ascorbate
|
Properties: Defined component, Simple component, Organic compound |
Solutions
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Trace minerals (see Medium [M142])
Dose: 10 G_PER_L
Cross-reference to Medium M142
10% Glucose solution
Dose: 25 G_PER_L
Role: Carbon source; Properties: Defined component, Organic compound, Solution
Thioglycolate--ascorbate solution* (see below)
Dose: 25 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M809_Modified_Haloanaerobium_Medium (CultureMech:010221)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.757620Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M809 |
| 2026-01-28T19:48:29.157392Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:48.362147+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:19.383079Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:12.222016Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010221 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.163011+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Haloanaerobium Medium" to "modified_haloanaerobium_medium" for consistent matching |
| 2026-05-16T09:03:21.070047+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.655429+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:33.170503+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-06T03:05:19.516120+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e4454a19e1f41038e9c6751fd6c1736b8dd876b378a74c5abbde89619b645b55 |