Overview
- External ontology
- TOGO:M844 — Spirochaeta Stenostrepta Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M844 Original source: JCM - JCM_M809 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=809
Composition
flowchart LR
CultureMech_010259["spirochaeta_stenostrepta_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010259 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Resazurin["Resazurin"]
CultureMech_010259 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_Thioglycolate["Thioglycolate"]
CultureMech_010259 --> ing2_Thioglycolate
CHEBI_47869["CHEBI:47869"]
ing2_Thioglycolate -.-> CHEBI_47869
ing3_Glucose["Glucose"]
CultureMech_010259 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010259 --> ing4_Yeast_extract__BD_Difco_
ing5_Peptone__BD_Difco_["Peptone (BD-Difco)"]
CultureMech_010259 --> ing5_Peptone__BD_Difco_
ing6_agar["agar"]
CultureMech_010259 --> ing6_agar
CHEBI_2509["CHEBI:2509"]
ing6_agar -.-> CHEBI_2509
ing7_KOH["KOH"]
CultureMech_010259 --> ing7_KOH
CHEBI_32035["CHEBI:32035"]
ing7_KOH -.-> CHEBI_32035
ing8_N2["N2"]
CultureMech_010259 --> ing8_N2
CHEBI_17997["CHEBI:17997"]
ing8_N2 -.-> CHEBI_17997
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 3 |
Thioglycolate
C2H2O2S 90.103 g/mol
|
0.5 G_PER_L |
CHEBI:47869
thioglycolate(2-)
|
Role: Reducing agent; Properties: Organic compound, Simple component, Defined component |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 5 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 6 | Peptone (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 7 | agar | variable VARIABLE |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 9 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M844_Spirochaeta_Stenostrepta_Medium (CultureMech:010259)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.816171Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M844 |
| 2026-01-28T19:48:36.729223Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: agar; Added default concentration for ingredient: KOH; Added default concentration for ingredient: N2 |
| 2026-03-13T06:20:12.479455Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010259 |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.423897+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Spirochaeta Stenostrepta Medium" to "spirochaeta_stenostrepta_medium" for consistent matching |
| 2026-05-16T09:03:21.335409+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:44.960293+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:19.764131+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c348553837dbda62797f79acf5d616d97f84d8f75fd52d27573fb07036048d5b |