spirochaeta_stenostrepta_medium

CultureMech:010259 bacterial COMPLEX SOLID_AGAR
Original name: Spirochaeta Stenostrepta Medium

Overview

External ontology
TOGO:M844 — Spirochaeta Stenostrepta Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M844 Original source: JCM - JCM_M809 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=809

Composition

flowchart LR
    CultureMech_010259["spirochaeta_stenostrepta_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010259 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Resazurin["Resazurin"]
    CultureMech_010259 --> ing1_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing1_Resazurin -.-> CHEBI_8806
    ing2_Thioglycolate["Thioglycolate"]
    CultureMech_010259 --> ing2_Thioglycolate
    CHEBI_47869["CHEBI:47869"]
    ing2_Thioglycolate -.-> CHEBI_47869
    ing3_Glucose["Glucose"]
    CultureMech_010259 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010259 --> ing4_Yeast_extract__BD_Difco_
    ing5_Peptone__BD_Difco_["Peptone (BD-Difco)"]
    CultureMech_010259 --> ing5_Peptone__BD_Difco_
    ing6_agar["agar"]
    CultureMech_010259 --> ing6_agar
    CHEBI_2509["CHEBI:2509"]
    ing6_agar -.-> CHEBI_2509
    ing7_KOH["KOH"]
    CultureMech_010259 --> ing7_KOH
    CHEBI_32035["CHEBI:32035"]
    ing7_KOH -.-> CHEBI_32035
    ing8_N2["N2"]
    CultureMech_010259 --> ing8_N2
    CHEBI_17997["CHEBI:17997"]
    ing8_N2 -.-> CHEBI_17997

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component
2 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component
3 Thioglycolate
C2H2O2S 90.103 g/mol
0.5 G_PER_L CHEBI:47869
thioglycolate(2-)
 Role: Reducing agent; Properties: Organic compound, Simple component, Defined component
4 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Defined component, Simple component, Organic compound
5 Yeast extract (BD-Difco) 2 G_PER_L Properties: Undefined component
6 Peptone (BD-Difco) 2 G_PER_L Properties: Undefined component
7 agar variable VARIABLE CHEBI:2509
agar
 Role: Solidifying component
8 KOH
HO.K 56.105 g/mol
variable VARIABLE CHEBI:32035
potassium hydroxide
 Role: Mineral source; Properties: Defined component, Solution
9 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.816171Z togo-import Imported from TOGO Medium Source: JCM, ID: M844
2026-01-28T19:48:36.729223Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: agar; Added default concentration for ingredient: KOH; Added default concentration for ingredient: N2
2026-03-13T06:20:12.479455Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010259
2026-03-17T06:30:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.423897+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Spirochaeta Stenostrepta Medium" to "spirochaeta_stenostrepta_medium" for consistent matching
2026-05-16T09:03:21.335409+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:44.960293+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.