Overview
- External ontology
- TOGO:M848 — LB (Luria-Bertani) Broth With 1 mM DTT
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M848 Original source: JCM - JCM_M813 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=813
Composition
flowchart LR
CultureMech_010263["lb_luria_bertani_broth_with_1_mm_dtt"]
ing0_Distilled_water["Distilled water"]
CultureMech_010263 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010263 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010263 --> ing2_Yeast_extract__BD_Difco_
ing3_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010263 --> ing3_Tryptone__BD_Difco_
ing4_1_4__dithiothreitol__DTT_["1,4--dithiothreitol (DTT)"]
CultureMech_010263 --> ing4_1_4__dithiothreitol__DTT_
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 4 | Tryptone (BD-Difco) | 10 G_PER_L | Properties: Undefined component | |
| 5 | 1,4--dithiothreitol (DTT) | variable VARIABLE | Properties: Defined component, Inorganic compound, Simple component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M848_LB_Luria-Bertani_Broth_With_1_mM_DTT (CultureMech:010263)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.824022Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M848 |
| 2026-01-28T19:48:09.215446Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 1,4--dithiothreitol (DTT) |
| 2026-03-13T06:20:12.508875Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010263 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.453028+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB (Luria-Bertani) Broth With 1 mM DTT" to "lb_luria_bertani_broth_with_1_mm_dtt" for consistent matching |
| 2026-05-16T09:03:21.358060+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:44.986345+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:19.782006+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f24f8fdcc4c5fe7a10e320657b80ae94c101788e63e5e0602c3acd16d1c69f74 |