lb_luria_bertani_broth_with_1_mm_dtt

Overview

External ontology

TOGO:M848 — LB (Luria-Bertani) Broth With 1 mM DTT

Applications

• Microbial cultivation

pH range

{'min': 7.0, 'max': 7.0}

Notes

MediaDive J813 describes JCM Medium 813 LB (Luria-Bertani) Broth with 1 mM DTT as 10.0 g/L BD-Difco tryptone, 5.0 g/L BD-Difco yeast extract, 10.0 g/L NaCl, and 1.0 mM final 1,4-dithiothreitol in 1000.0 ml/L distilled water, adjusted to pH 7.0.

Composition

flowchart LR
    CultureMech_010263["lb_luria_bertani_broth_with_1_mm_dtt"]
    ing0_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_010263 --> ing0_Tryptone__BD_Difco_
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010263 --> ing1_Yeast_extract__BD_Difco_
    ing2_NaCl["NaCl"]
    CultureMech_010263 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Distilled_water["Distilled water"]
    CultureMech_010263 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377
    ing4_1_4_dithiothreitol__DTT_["1,4-dithiothreitol (DTT)"]
    CultureMech_010263 --> ing4_1_4_dithiothreitol__DTT_
    CHEBI_18320["CHEBI:18320"]
    ing4_1_4_dithiothreitol__DTT_ -.-> CHEBI_18320

Ingredients (5)

Preparation

1. Dissolve 10.0 g/L BD-Difco tryptone, 5.0 g/L BD-Difco yeast extract, and 10.0 g/L NaCl in 1000.0 ml/L distilled water.
2. Adjust pH to 7.0.
3. Autoclave the pH-adjusted basal LB broth.
4. After autoclaving, aseptically add 1.0 mM final 1,4-dithiothreitol (DTT).

Target organisms

No target organisms are recorded.

Provenance