koko_medium

CultureMech:010277 bacterial COMPLEX LIQUID
Original name: KOKO Medium

Overview

External ontology
TOGO:M860 — KOKO Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M860 Original source: JCM - JCM_M825 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=825

Composition

flowchart LR
    CultureMech_010277["koko_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010277 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_010277 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_010277 --> ing2_Yeast_extract
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010277 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_010277 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_K2HPO4["K2HPO4"]
    CultureMech_010277 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Resazurin["Resazurin"]
    CultureMech_010277 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_NaH2PO4_2H2O["NaH2PO4・2H2O"]
    CultureMech_010277 --> ing7_NaH2PO4_2H2O
    CHEBI_37585["CHEBI:37585"]
    ing7_NaH2PO4_2H2O -.-> CHEBI_37585
    ing8_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_010277 --> ing8_Tryptone__BD_Difco_
    ing9_Peptone["Peptone"]
    CultureMech_010277 --> ing9_Peptone
    ing10_N2["N2"]
    CultureMech_010277 --> ing10_N2
    CHEBI_17997["CHEBI:17997"]
    ing10_N2 -.-> CHEBI_17997

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
930 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.16 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 Yeast extract 1 G_PER_L Role: Nutrient source; Properties: Undefined component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.06 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
6 K2HPO4
HO4P.2K 174.174 g/mol
1.6 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
7 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component
8 NaH2PO4・2H2O
H2O4P.Na 119.976 g/mol
1 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
9 Tryptone (BD-Difco) 1 G_PER_L Properties: Undefined component
10 Peptone 1 G_PER_L
11 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas

Solutions

SL--4 trace element solution (see Medium [M335])

Dose: 10 G_PER_L

Cross-reference to Medium M335

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

8% NaHCO3 solution*

Dose: 1.25 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

10% Glucose solution

Dose: 50 G_PER_L

Role: Carbon source; Properties: Defined component, Organic compound, Solution

3% Na2S・9H2O solution

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component

3% L--Cysteine・HCl・H2O solution

Dose: 10 G_PER_L

Properties: Defined component, Organic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.850524Z togo-import Imported from TOGO Medium Source: JCM, ID: M860
2026-01-28T19:48:28.070620Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:46.467512+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 6 solution(s) from ingredients
2026-03-13T06:20:12.625407Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010277
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:24.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.572145+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "KOKO Medium" to "koko_medium" for consistent matching
2026-05-16T09:03:21.484461+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:45.117760+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:33.536608+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:51.855326+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:19.940288+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 6ef221136714aa049a31b4f3b512adb5ea7e0bf3a34eabc5aa870a42523a64ed