koko_medium

Overview

External ontology

TOGO:M860 — KOKO Medium

Applications

• Microbial cultivation

pH range

{'min': 7.0, 'max': 7.0}

Notes

JCM Medium 825 / KOKO Medium lists 930.0 ml/L distilled water, 0.16 g/L MgSO4 x 7 H2O, 1.0 g/L yeast extract, 0.06 g/L CaCl2 x 2 H2O, 0.5 g/L NH4Cl, 1.6 g/L K2HPO4, 0.5 mg/L resazurin, 1.0 g/L NaH2PO4 x 2 H2O, 1.0 g/L BD-Difco tryptone, 1.0 g/L peptone, 10.0 ml/L SL-4 trace element solution, 10.0 ml/L Trace vitamins, N2 gas, and sterile anaerobic 8% NaHCO3, 10% glucose, 3% Na2S x 9 H2O, and 3% L-cysteine HCl x H2O stocks; MediaDive J825 reports the same recipe at pH 7.0.

Composition

flowchart LR
    CultureMech_010277["koko_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010277 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_010277 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_010277 --> ing2_Yeast_extract
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_010277 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_010277 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_K2HPO4["K2HPO4"]
    CultureMech_010277 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Resazurin["Resazurin"]
    CultureMech_010277 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
    CultureMech_010277 --> ing7_NaH2PO4_x_2_H2O
    CHEBI_37585["CHEBI:37585"]
    ing7_NaH2PO4_x_2_H2O -.-> CHEBI_37585
    ing8_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_010277 --> ing8_Tryptone__BD_Difco_
    ing9_Peptone["Peptone"]
    CultureMech_010277 --> ing9_Peptone
    ing10_N2["N2"]
    CultureMech_010277 --> ing10_N2
    CHEBI_17997["CHEBI:17997"]
    ing10_N2 -.-> CHEBI_17997

Ingredients (11)

Preparation

1. Mix the basal salts, yeast extract, BD-Difco tryptone, peptone, resazurin, 10.0 ml/L Trace element solution SL-4, 10.0 ml/L Trace vitamins, and 930.0 ml/L distilled water.
2. Adjust the medium to pH 7.0.
3. Autoclave the medium under an N2 atmosphere.
4. After autoclaving, add 1.25 ml/L sterile anaerobic 8% NaHCO3 solution, 50.0 ml/L 10% glucose solution, 10.0 ml/L 3% Na2S x 9 H2O solution, and 10.0 ml/L 3% L-cysteine HCl x H2O solution.

Solutions

Target organisms

No target organisms are recorded.

Provenance