Overview
- External ontology
- TOGO:M970 — Peat Medium 2 For Methanobacteria
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M970 Original source: JCM - JCM_M924 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=924
Composition
flowchart LR
CultureMech_010396["peat_medium_2_for_methanobacteria"]
ing0_Distilled_water["Distilled water"]
CultureMech_010396 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010396 --> ing1_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing1_Carbon_dioxide_gas -.-> CHEBI_16526
ing2_Nitrogen_gas["Nitrogen gas"]
CultureMech_010396 --> ing2_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing2_Nitrogen_gas -.-> CHEBI_17997
ing3_10_mM_Sodium_acetate["10 mM Sodium acetate"]
CultureMech_010396 --> ing3_10_mM_Sodium_acetate
ing4_Hydrogen_gas["Hydrogen gas"]
CultureMech_010396 --> ing4_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing4_Hydrogen_gas -.-> CHEBI_18276
ing5_EDTA_2Na["EDTA・2Na"]
CultureMech_010396 --> ing5_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing5_EDTA_2Na -.-> CHEBI_64734
ing6_NaOH["NaOH"]
CultureMech_010396 --> ing6_NaOH
CHEBI_32145["CHEBI:32145"]
ing6_NaOH -.-> CHEBI_32145
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 3 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 4 | 10 mM Sodium acetate | 0.015 G_PER_L | Role: Buffer; Properties: Defined component, Organic compound, Solution | |
| 5 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 6 |
EDTA・2Na
C10H14N2O8.2Na 336.208 g/mol
|
37.23 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 7 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Solutions
Major metals (see Medium [M969])
Dose: 10 G_PER_L
Cross-reference to Medium M969
Trace metal 2 solution (see below)
Dose: 1 G_PER_L
1% Yeast extract solution
Dose: 0.01 G_PER_L
Role: Nutrient source; Properties: Undefined component
50 mM Coenzyme M solution
Dose: 0.025 G_PER_L
Role: Growth factor; Properties: Defined component, Organic compound, Solution
4 mM Na2S・9H2O solution*
Dose: 0.05 G_PER_L
Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component
1.0 M MES solution* (pH 7.45)
Dose: 0.1 G_PER_L
Role: Buffer; Properties: Organic compound, Solution, Defined component
83 mM TiNTA solution (see Medium [M969])
Dose: 0.05 G_PER_L
Cross-reference to Medium M969
Vitamin solution (see Medium [M969])
Dose: 0.05 G_PER_L
Cross-reference to Medium M969
Trace metal 1 solution (see Medium [M969])
Dose: 1 G_PER_L
Cross-reference to Medium M969
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M970_Peat_Medium_2_For_Methanobacteria (CultureMech:010396)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.057516Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M970 |
| 2026-01-28T19:48:30.976622Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:51.533813+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 9 solution(s) from ingredients |
| 2026-03-13T03:09:22.890268Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:13.537981Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010396 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.511218+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Peat Medium 2 For Methanobacteria" to "peat_medium_2_for_methanobacteria" for consistent matching |
| 2026-05-16T09:03:22.420891+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:46.142518+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:27.949692+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f728dc49c836071034e6efc5836562a6e2da5c08b72384f40855867d449a50d3 |