Overview
- External ontology
- TOGO:M970 — Peat Medium 2 For Methanobacteria
- Applications
-
- Microbial cultivation
- pH
- 5.7
- Notes
- JCM Medium 924 prepares Peat Medium 2 For Methanobacteria from Major metals and TiNTA/Vitamin subrecipes in JCM 923 plus a JCM 924 Trace metal 2 stock; the culture-tube additions are scaled from per-5-ml additions to ml/L.
Composition
flowchart LR
CultureMech_010396["peat_medium_2_for_methanobacteria"]
ing0_Distilled_water["Distilled water"]
CultureMech_010396 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_N2["N2"]
CultureMech_010396 --> ing1_N2
CHEBI_17997["CHEBI:17997"]
ing1_N2 -.-> CHEBI_17997
ing2_CO2["CO2"]
CultureMech_010396 --> ing2_CO2
CHEBI_16526["CHEBI:16526"]
ing2_CO2 -.-> CHEBI_16526
ing3_H2["H2"]
CultureMech_010396 --> ing3_H2
CHEBI_18276["CHEBI:18276"]
ing3_H2 -.-> CHEBI_18276
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
JCM Medium 924 lists 1.0 L Distilled water. |
| 2 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 924 uses N2-CO2 (80:20, v/v) during cooling and dispensing. |
| 3 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
JCM Medium 924 uses N2-CO2 during cooling and H2-CO2 during pressurization. |
| 4 |
H2
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
JCM Medium 924 pressurizes the tubes to 70 kPa H2-CO2 (80:20, v/v). |
Preparation
- Mix Major metals, Trace metal 2 solution, and distilled water thoroughly.
- Boil for 5 minutes, cool under N2-CO2, dispense under the same gas mixture, seal, and autoclave.
- For each 5 ml tube, add sterile anaerobic TiNTA, MES, vitamin, yeast extract, Coenzyme M, sodium acetate, and Na2S stocks.
- Pressurize tubes to 70 kPa H2-CO2 (80:20, v/v), stand at least 7 hr, and check final pH at about 5.7.
Solutions
Major metals
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| KCl | 0.15 G_PER_L | JCM Medium 923 prints this component in its stock solution. |
| KH2PO4 | 1.36 G_PER_L | JCM Medium 923 prints this component in its stock solution. |
| NH4Cl | 2.68 G_PER_L | JCM Medium 923 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 923 prints this component in its stock solution. |
JCM Medium 924 adds 10.0 ml/L Major metals from JCM Medium 923.
Trace metal 2 solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CoCl2 x 6H2O | 24.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| ZnCl2 | 75.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| H3BO3 | 19.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| NiCl2 x 6H2O | 24.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| Na2MoO4 x 2H2O | 24.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| FeCl2 x 4H2O | 1.344 G_PER_L | JCM Medium 923 prints this component in its stock solution. |
| MnSO4 x H2O | 26.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| MgSO4 x 7H2O | 1.556 G_PER_L | JCM Medium 923 prints this component in its stock solution. |
| CaCl2 x 2H2O | 2.336 G_PER_L | JCM Medium 923 prints this component in its stock solution. |
| CuSO4 x 5H2O | 9.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| AlK(SO4)2 x 12H2O | 3.446 G_PER_L | JCM Medium 923 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 923 prints this component in its stock solution. |
| EDTA x 2Na | 37.23 G_PER_L | JCM Medium 924 prints this component in its stock solution. |
| NaOH | variable VARIABLE | JCM Medium 924 adjusts Trace metal 2 solution to pH 7.0 with NaOH. |
JCM Medium 924 adds 1.0 ml/L Trace metal 2 solution.
83 mM TiNTA solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| 1 M Tris base (pH 8) | variable VARIABLE | JCM Medium 923 prints 7.2 ml 1 M Tris base (pH 8) in its 83 mM TiNTA stock; no final stock volume is stated. |
| 0.5 M Nitrilotriacetic acid, disodium salt | variable VARIABLE | JCM Medium 923 prints 4.8 ml 0.5 M Nitrilotriacetic acid, disodium salt in its 83 mM TiNTA stock; no final stock volume is stated. |
| 15% TiCl3 solution | variable VARIABLE | JCM Medium 923 prints 0.55 ml 15% TiCl3 solution in its 83 mM TiNTA stock; no final stock volume is stated. |
JCM Medium 924 adds 0.05 ml 83 mM TiNTA solution to each 5 ml tube; this is 10 ml/L.
1.0 M MES solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MES | 1.0 MOLAR | JCM Medium 924 identifies this as a 1.0 M stock solution. |
JCM Medium 924 adds 0.10 ml 1.0 M MES solution to each 5 ml tube; this is 20 ml/L.
Vitamin solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 20.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| Folic acid | 20.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| Pyridoxine HCl | 100.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| Thiamine HCl | 50.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| Riboflavin | 50.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| Nicotinic acid | 50.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| DL-Calcium pantothenate | 50.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| Vitamin B12 | 1.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| p-Aminobenzoic acid | 50.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| Lipoic acid | 50.0 MG_PER_L | JCM Medium 923 prints this component in its stock solution. |
| Distilled water | 1.0 L | JCM Medium 923 prints this component in its stock solution. |
JCM Medium 924 adds 0.05 ml Vitamin solution from JCM Medium 923 to each 5 ml tube; this is 10 ml/L.
1% Yeast extract solution
Dose: 2.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 1 PERCENT_W_V | JCM Medium 924 identifies this as a 1% w/v stock solution. |
JCM Medium 924 adds 0.01 ml 1% Yeast extract solution to each 5 ml tube; this is 2 ml/L.
50 mM Coenzyme M solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Coenzyme M | 0.05 MOLAR | JCM Medium 924 identifies this as a 0.05 M stock solution. |
JCM Medium 924 adds 0.025 ml 50 mM Coenzyme M solution to each 5 ml tube; this is 5 ml/L.
10 mM Sodium acetate
Dose: 3.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium acetate | 10 MILLIMOLAR | JCM Medium 924 identifies this as a 10 mM stock solution. |
JCM Medium 924 adds 0.015 ml 10 mM Sodium acetate to each 5 ml tube; this is 3 ml/L.
4 mM Na2S x 9H2O solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 4 MILLIMOLAR | JCM Medium 924 identifies this as a 4 mM stock solution. |
JCM Medium 924 adds 0.05 ml 4 mM Na2S x 9H2O solution to each 5 ml tube; this is 10 ml/L.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.057516Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M970 |
| 2026-01-28T19:48:30.976622Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:51.533813+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 9 solution(s) from ingredients |
| 2026-03-13T03:09:22.890268Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:13.537981Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010396 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.511218+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Peat Medium 2 For Methanobacteria" to "peat_medium_2_for_methanobacteria" for consistent matching |
| 2026-05-16T09:03:22.420891+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:46.142518+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_togo_jcm_archaea_score15.py | RESOLVED_TOGO_JCM_ARCHAEA_SCORE15 | JCM Medium 924 prepares Peat Medium 2 For Methanobacteria from Major metals and TiNTA/Vitamin subrecipes in JCM 923 plus a JCM 924 Trace metal 2 stock; the culture-tube additions are scaled from per-5-ml additions to ml/L. |