mrs_medium_with_2_5_nacl_ph_9_0

CultureMech:010403 bacterial COMPLEX SOLID_AGAR
Original name: MRS Medium With 2.5% NaCl (pH 9.0)

Overview

External ontology
TOGO:M977 — MRS Medium With 2.5% NaCl (pH 9.0)
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'mrs_medium_with_10_nacl', 'source': 'TOGO', 'source_id': 'TOGO:M249', 'original_category': 'bacterial'}
  • {'name': 'mrs_medium_with_2_5_nacl', 'source': 'TOGO', 'source_id': 'TOGO:M602', 'original_category': 'bacterial'}
Notes
Source: https://togomedium.org/medium/M977 Original source: JCM - JCM_M931 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=931

Composition

flowchart LR
    CultureMech_010403["mrs_medium_with_2_5_nacl_ph_9_0"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010403 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_010403 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
    CultureMech_010403 --> ing2_Bacto_agar__BD_Difco_
    ing3_Lactobacilli_MRS_broth__BD_Difco_["Lactobacilli MRS broth (BD-Difco)"]
    CultureMech_010403 --> ing3_Lactobacilli_MRS_broth__BD_Difco_

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 Bacto agar (BD-Difco) 15 G_PER_L Role: Solidifying component
4 Lactobacilli MRS broth (BD-Difco) 55 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:56.071138Z togo-import Imported from TOGO Medium Source: JCM, ID: M977
2026-01-28T19:48:18.100526Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:13.595746Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010403
2026-03-17T06:30:03.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.568877+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MRS Medium With 2.5% NaCl (pH 9.0)" to "mrs_medium_with_2_5_nacl_ph_9_0" for consistent matching
2026-06-05T07:40:46.208991+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:12.096678+00:00 recipe-merger Merged 3 duplicate recipes into canonical record Sources: TOGO_M249_MRS_Medium_With_10_NaCl, TOGO_M602_MRS_Medium_With_2.5_NaCl, TOGO_M977_MRS_Medium_With_2.5_NaCl_pH_9.0
2026-08-06T03:05:12.096728+00:00 merge_recipes.py MERGED_RECIPES merged 3 source recipe(s) on fingerprint 8527b26e09d54b728f00cb5cce2d246716e114972bd48eb385e1d28bcd430462