Overview
- External ontology
- TOGO:M977 — MRS Medium With 2.5% NaCl (pH 9.0)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M977 Original source: JCM - JCM_M931 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=931
Composition
flowchart LR
CultureMech_010403["mrs_medium_with_2_5_nacl_ph_9_0"]
ing0_Distilled_water["Distilled water"]
CultureMech_010403 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010403 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
CultureMech_010403 --> ing2_Bacto_agar__BD_Difco_
ing3_Lactobacilli_MRS_broth__BD_Difco_["Lactobacilli MRS broth (BD-Difco)"]
CultureMech_010403 --> ing3_Lactobacilli_MRS_broth__BD_Difco_
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
25 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 | Bacto agar (BD-Difco) | 15 G_PER_L | Role: Solidifying component | |
| 4 | Lactobacilli MRS broth (BD-Difco) | 55 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.071138Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M977 |
| 2026-01-28T19:48:18.100526Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:13.595746Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010403 |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.568877+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MRS Medium With 2.5% NaCl (pH 9.0)" to "mrs_medium_with_2_5_nacl_ph_9_0" for consistent matching |
| 2026-06-05T07:40:46.208991+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |