desulfurivibrio_ames2_medium

Overview

External ontology

jcm.grmd:1333 (source link unavailable) — DESULFURIVIBRIO AMeS2 MEDIUM

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1333

Composition

flowchart LR
    CultureMech_015831["desulfurivibrio_ames2_medium"]
    ing0_Na2CO3["Na2CO3"]
    CultureMech_015831 --> ing0_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3 -.-> CHEBI_29377
    ing1_NaHCO3["NaHCO3"]
    CultureMech_015831 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_NaCl["NaCl"]
    CultureMech_015831 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_015831 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Sulfur["Sulfur"]
    CultureMech_015831 --> ing4_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing4_Sulfur -.-> CHEBI_26833
    ing5_1_M_MgCl2_solution["1 M MgCl2 solution"]
    CultureMech_015831 --> ing5_1_M_MgCl2_solution
    ing6_1_M_NH4Cl_solution["1 M NH4Cl solution"]
    CultureMech_015831 --> ing6_1_M_NH4Cl_solution
    ing7_Trace_element_solution__see_Medium_No__1079__["Trace element solution (see Medium No. 1079 )"]
    CultureMech_015831 --> ing7_Trace_element_solution__see_Medium_No__1079__
    ing8_Se_W_solution___see_Medium_No__852__["Se/W solution* (see Medium No. 852 )"]
    CultureMech_015831 --> ing8_Se_W_solution___see_Medium_No__852__
    ing9_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
    CultureMech_015831 --> ing9_Trace_vitamins___see_Medium_No__197__
    ing10_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015831 --> ing10_5__Na2S_9H2O_solution

Ingredients (11)

Preparation

1. Add components, except sulfur, to distilled water and bring volume to 1.0 L.
2. Autoclave in closed bottles.
3. After cooling, aseptically add per liter the following solutions (autoclaved or *filter-sterilized): Steam sulfur for 3 hr on each of 3 successive days.
4. Aseptically and anaerobically distribute the medium and sterilized sulfur into culture vessels (e.g., 10 ml medium and 0.05 g sulfur in Hungate tubes) under a N2 gas steam and seal with butyl rubber stoppers.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J1333_DESULFURIVIBRIO_AMeS2_MEDIUM (CultureMech:015831)