natronoarchaea_medium_ii

Overview

External ontology

jcm.grmd:1334 (source link unavailable) — NATRONOARCHAEA MEDIUM II

Applications

• Microbial cultivation

pH

7.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1334

Composition

flowchart LR
    CultureMech_015832["natronoarchaea_medium_ii"]
    ing0_Neutral_base_salt_medium__see_below_["Neutral base salt medium (see below)"]
    CultureMech_015832 --> ing0_Neutral_base_salt_medium__see_below_
    ing1_Base_soda_medium__see_Medium_No__1207__["Base soda medium (see Medium No. 1207 )"]
    CultureMech_015832 --> ing1_Base_soda_medium__see_Medium_No__1207__
    ing2_1_M_MgCl2_solution["1 M MgCl2 solution"]
    CultureMech_015832 --> ing2_1_M_MgCl2_solution
    ing3_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
    CultureMech_015832 --> ing3_Trace_vitamins___see_Medium_No__197__
    ing4_Trace_element_solution__see_Medium_No__1079__["Trace element solution (see Medium No. 1079 )"]
    CultureMech_015832 --> ing4_Trace_element_solution__see_Medium_No__1079__
    ing5_Se_W_solution__see_Medium_No__852__["Se/W solution (see Medium No. 852 )"]
    CultureMech_015832 --> ing5_Se_W_solution__see_Medium_No__852__
    ing6_10__Yeast_extract_solution["10% Yeast extract solution"]
    CultureMech_015832 --> ing6_10__Yeast_extract_solution
    ing7_10__Soluble_starch_solution["10% Soluble starch solution"]
    CultureMech_015832 --> ing7_10__Soluble_starch_solution
    ing8_NaCl["NaCl"]
    CultureMech_015832 --> ing8_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing8_NaCl -.-> CHEBI_26710
    ing9_KCl["KCl"]
    CultureMech_015832 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10_K2HPO4["K2HPO4"]
    CultureMech_015832 --> ing10_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing10_K2HPO4 -.-> CHEBI_131527
    ing11_NH4Cl["NH4Cl"]
    CultureMech_015832 --> ing11_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing11_NH4Cl -.-> CHEBI_31206
    ing12__NH4_2SO4["(NH4)2SO4"]
    CultureMech_015832 --> ing12__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing12__NH4_2SO4 -.-> CHEBI_62946

Ingredients (13)

Preparation

1. Mix two media autoclaved separately.
2. Aseptically add the following solutions (autoclaved or *filter-sterilized): Neutral base salt medium: Add components to distilled water and bring volume to 1.0 L.
3. Adjust pH to 7.0 with 0.5 M K2HPO4 and autoclave.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J1334_NATRONOARCHAEA_MEDIUM_II (CultureMech:015832)