atribacterota_m15_medium

Overview

External ontology

jcm.grmd:1392 (source link unavailable) — ATRIBACTEROTA M15 MEDIUM

Applications

• Microbial cultivation

pH

7.5

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1392

Composition

flowchart LR
    CultureMech_015850["atribacterota_m15_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_015850 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015850 --> ing1_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_6H2O -.-> CHEBI_86345
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015850 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_015850 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_KH2PO4["KH2PO4"]
    CultureMech_015850 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_Trace_element_solution__see_Medium_No__439__["Trace element solution (see Medium No. 439 )"]
    CultureMech_015850 --> ing5_Trace_element_solution__see_Medium_No__439__
    ing6_Resazurin["Resazurin"]
    CultureMech_015850 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Distilled_water["Distilled water"]
    CultureMech_015850 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377
    ing8_Selenite_tungstate_solution__see_Medium_No__431__["Selenite-tungstate solution (see Medium No. 431 )"]
    CultureMech_015850 --> ing8_Selenite_tungstate_solution__see_Medium_No__431__
    ing9_8__NaHCO3_solution["8% NaHCO3 solution"]
    CultureMech_015850 --> ing9_8__NaHCO3_solution
    ing10_Vitamin_solution__see_Medium_No__403__["Vitamin solution (see Medium No. 403 )"]
    CultureMech_015850 --> ing10_Vitamin_solution__see_Medium_No__403__
    ing11_Thiamine_solution__see_Medium_No__403__["Thiamine solution (see Medium No. 403 )"]
    CultureMech_015850 --> ing11_Thiamine_solution__see_Medium_No__403__
    ing12_Vitamin_B12_solution__see_Medium_No__403__["Vitamin B12 solution (see Medium No. 403 )"]
    CultureMech_015850 --> ing12_Vitamin_B12_solution__see_Medium_No__403__
    ing13_10__Yeast_extract_solution["10% Yeast extract solution"]
    CultureMech_015850 --> ing13_10__Yeast_extract_solution
    ing14_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015850 --> ing14_5__Na2S_9H2O_solution

Ingredients (15)

Preparation

1. Mix components thoroughly and adjust pH 7.5.
2. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream.
3. Autoclave under the same gas mixture.
4. After cooling, add the following solutions (filter-sterilized): Aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml medium in Balch tubes) under the same gas mixture and seal with butyl rubber stoppers.
5. Add per liter the following solution (autoclave or *filter-sterilize and store under a N2 atmosphere).
6. Readjust pH to 7.5-8.0, if necessary.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J1392_ATRIBACTEROTA_M15_MEDIUM (CultureMech:015850)