artificial_brackish_water_medium_2

Overview

External ontology

jcm.grmd:1399 (source link unavailable) — ARTIFICIAL BRACKISH WATER MEDIUM 2

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1399

Composition

flowchart LR
    CultureMech_015853["artificial_brackish_water_medium_2"]
    ing0_NaCl["NaCl"]
    CultureMech_015853 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KH2PO4["KH2PO4"]
    CultureMech_015853 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_015853 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_KCl["KCl"]
    CultureMech_015853 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Na2SO4["Na2SO4"]
    CultureMech_015853 --> ing4_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing4_Na2SO4 -.-> CHEBI_32149
    ing5_Trace_element_solution__see_Medium_No__439__["Trace element solution (see Medium No. 439 )"]
    CultureMech_015853 --> ing5_Trace_element_solution__see_Medium_No__439__
    ing6_Selenite_tungstate_solution__see_Medium_No__431__["Selenite tungstate solution (see Medium No. 431 )"]
    CultureMech_015853 --> ing6_Selenite_tungstate_solution__see_Medium_No__431__
    ing7_Resazurin["Resazurin"]
    CultureMech_015853 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_Distilled_water["Distilled water"]
    CultureMech_015853 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377
    ing9_8__NaHCO3_solution_["8% NaHCO3 solution*"]
    CultureMech_015853 --> ing9_8__NaHCO3_solution_
    ing10_1_M_MgCl2_6H2O_solution["1 M MgCl2·6H2O solution"]
    CultureMech_015853 --> ing10_1_M_MgCl2_6H2O_solution
    ing11_1_M_CaCl2_2H2O_solution["1 M CaCl2·2H2O solution"]
    CultureMech_015853 --> ing11_1_M_CaCl2_2H2O_solution
    ing12_Vitamin_solution__see_Medium_No__403___["Vitamin solution (see Medium No. 403 )*"]
    CultureMech_015853 --> ing12_Vitamin_solution__see_Medium_No__403___
    ing13_Thiamine_solution__see_Medium_No__403___["Thiamine solution (see Medium No. 403 )*"]
    CultureMech_015853 --> ing13_Thiamine_solution__see_Medium_No__403___
    ing14_Vitamin_B12_solution__see_Medium_No__403___["Vitamin B12 solution (see Medium No. 403 )*"]
    CultureMech_015853 --> ing14_Vitamin_B12_solution__see_Medium_No__403___
    ing15_1_M_Sodium_formate_solution["1 M Sodium formate solution"]
    CultureMech_015853 --> ing15_1_M_Sodium_formate_solution
    ing16_1_M_Sodium_acetate_solution["1 M Sodium acetate solution"]
    CultureMech_015853 --> ing16_1_M_Sodium_acetate_solution
    ing17_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015853 --> ing17_5__Na2S_9H2O_solution

Ingredients (18)

Preparation

1. Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere.
2. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filtersterilized): Aseptically and anaerobically, distribute the medium into culture vessels under the same gas mixture (e.g., 20 ml in 60 ml-serum bottles) and seal with butyl rubber stoppers.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J1399_ARTIFICIAL_BRACKISH_WATER_MEDIUM_2 (CultureMech:015853)