Overview
- External ontology
- jcm.grmd:1415 (source link unavailable) — METHANOSARCINA Z-7115 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1415
Composition
flowchart LR
CultureMech_015858["methanosarcina_z_7115_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_015858 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_015858 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_015858 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_015858 --> ing3_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_6H2O -.-> CHEBI_86345
ing4_Sodium_acetate["Sodium acetate"]
CultureMech_015858 --> ing4_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Sodium_acetate -.-> CHEBI_32954
ing5_Trace_element_solution__see_Medium_No__899__["Trace element solution (see Medium No. 899 )"]
CultureMech_015858 --> ing5_Trace_element_solution__see_Medium_No__899__
ing6_Resazurin["Resazurin"]
CultureMech_015858 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Distilled_water["Distilled water"]
CultureMech_015858 --> ing7_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing7_Distilled_water -.-> CHEBI_15377
ing8_8__NaHCO3_soluiton_["8% NaHCO3 soluiton*"]
CultureMech_015858 --> ing8_8__NaHCO3_soluiton_
ing9_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
CultureMech_015858 --> ing9_Trace_vitamins___see_Medium_No__197__
ing10_50___v_v__Methanol["50% (v/v) Methanol"]
CultureMech_015858 --> ing10_50___v_v__Methanol
ing11_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
CultureMech_015858 --> ing11_5__L_Cysteine_HCl_H2O_solution
ing12_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
CultureMech_015858 --> ing12_5__Na2S_9H2O_solution
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.16 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.33 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.2 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 6 | Trace element solution (see Medium No. 899 ) | 1.0 ML_PER_L | ||
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 MG_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
Distilled water
H2O 18.015 g/mol
|
960.0 ML_PER_L |
CHEBI:15377
water
|
|
| 9 | 8% NaHCO3 soluiton* | 25.0 ML_PER_L | ||
| 10 | Trace vitamins* (see Medium No. 197 ) | 10.0 ML_PER_L | ||
| 11 | 50% (v/v) Methanol | 4.0 ML_PER_L | ||
| 12 | 5% L-Cysteine·HCl·H2O solution | 5.0 ML_PER_L | ||
| 13 | 5% Na2S·9H2O solution | 5.0 ML_PER_L |
Preparation
- Mix components and adjust pH to 7.0.
- Autoclave the medium under a N2-CO2 (80:20 v/v) gas mixture.
- After cooling, aseptically and anaerobically, add the following solutions (autoclaved or *filter-sterilized): Dispense the medium into culture vessels (e.g., 20 ml of the medium in 60 ml serum bottles) under the same gas mixture and seal with butyl rubber stoppers.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1415_METHANOSARCINA_Z_7115_MEDIUM (CultureMech:015858)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-02T07:09:08.381209+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1415. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-06-03T02:00:03.372111Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-06-03T07:12:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-08-06T03:04:20.549853+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c98d30b03eebb998b420dde0bf62b6bc4a5b41a57b59788aa686a58328601a0c |