modified_methanobacterium_medium

Overview

External ontology

jcm.grmd:1427 (source link unavailable) — MODIFIED METHANOBACTERIUM MEDIUM

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1427

Composition

flowchart LR
    CultureMech_015863["modified_methanobacterium_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_015863 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015863 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_015863 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_015863 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015863 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_FeCl2_solution__see_Medium_No__187__["FeCl2 solution (see Medium No. 187 )"]
    CultureMech_015863 --> ing5_FeCl2_solution__see_Medium_No__187__
    ing6_Trace_element_solution__see_Medium_No__187__["Trace element solution (see Medium No. 187 )"]
    CultureMech_015863 --> ing6_Trace_element_solution__see_Medium_No__187__
    ing7_Brain_heart_infusion__BD_Difico_["Brain heart infusion (BD Difico)"]
    CultureMech_015863 --> ing7_Brain_heart_infusion__BD_Difico_
    ing8_Proteose_peptone__BD_Difico_["Proteose peptone (BD Difico)"]
    CultureMech_015863 --> ing8_Proteose_peptone__BD_Difico_
    ing9_Yeast_extract__Oxoid_["Yeast extract (Oxoid)"]
    CultureMech_015863 --> ing9_Yeast_extract__Oxoid_
    ing10_Sodium_acetate["Sodium acetate"]
    CultureMech_015863 --> ing10_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing10_Sodium_acetate -.-> CHEBI_32954
    ing11_Sodium_formate["Sodium formate"]
    CultureMech_015863 --> ing11_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing11_Sodium_formate -.-> CHEBI_62965
    ing12_Rumen_fluid__clarified__see_Medium_No__266__["Rumen fluid, clarified (see Medium No. 266 )"]
    CultureMech_015863 --> ing12_Rumen_fluid__clarified__see_Medium_No__266__
    ing13_Resazurin["Resazurin"]
    CultureMech_015863 --> ing13_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing13_Resazurin -.-> CHEBI_8806
    ing14_Distilled_water["Distilled water"]
    CultureMech_015863 --> ing14_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing14_Distilled_water -.-> CHEBI_15377
    ing15_Vitamin_solution__see_Medium_No__898__["Vitamin solution (see Medium No. 898 )"]
    CultureMech_015863 --> ing15_Vitamin_solution__see_Medium_No__898__
    ing16_8__NaHCO3_solution["8% NaHCO3 solution"]
    CultureMech_015863 --> ing16_8__NaHCO3_solution
    ing17_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015863 --> ing17_5__Na2S_9H2O_solution
    ing18_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
    CultureMech_015863 --> ing18_5__L_Cysteine_HCl_H2O_solution

Ingredients (19)

Preparation

1. Mix components and autoclave under a N2-CO2 (80:20, v/v) gas mixture.
2. After cooling, aseptically and anaerobically, add the following solutions from anaerobic stocks (filtersterilized): Dispense the medium into culture vessels (e.g., 5 ml medium in Hungate tubes) under a H2-CO2 (80:20, v/v) gas stream and seal with butyl stoppers.
3. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere): After inoculation, pressurize the inoculated culture vessels to 100 kPa H2-CO2 (80:20, v/v).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J1427_MODIFIED_METHANOBACTERIUM_MEDIUM (CultureMech:015863)