Overview
- External ontology
- jcm.grmd:1429 (source link unavailable) — M1H NAG ASW
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1429
Composition
flowchart LR
CultureMech_015864["m1h_nag_asw"]
ing0_HEPES["HEPES"]
CultureMech_015864 --> ing0_HEPES
CHEBI_46756["CHEBI:46756"]
ing0_HEPES -.-> CHEBI_46756
ing1_Peptone["Peptone"]
CultureMech_015864 --> ing1_Peptone
ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_015864 --> ing2_Yeast_extract__BD_Difco_
ing3_Wolfe_s_mineral_solution__see_Medium_No__265__["Wolfe's mineral solution (see Medium No. 265 )"]
CultureMech_015864 --> ing3_Wolfe_s_mineral_solution__see_Medium_No__265__
ing4_Modified_Hutner_s_basal_salts__see_Medium_No__900__["Modified Hutner's basal salts (see Medium No. 900 )"]
CultureMech_015864 --> ing4_Modified_Hutner_s_basal_salts__see_Medium_No__900__
ing5_Artificial_seawater["Artificial seawater"]
CultureMech_015864 --> ing5_Artificial_seawater
ing6_Distilled_water["Distilled water"]
CultureMech_015864 --> ing6_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing6_Distilled_water -.-> CHEBI_15377
ing7_Glucose["Glucose"]
CultureMech_015864 --> ing7_Glucose
CHEBI_17234["CHEBI:17234"]
ing7_Glucose -.-> CHEBI_17234
ing8_N__Acetyl_D_glucosamine["N -Acetyl-D-glucosamine"]
CultureMech_015864 --> ing8_N__Acetyl_D_glucosamine
ing9_Trace_vitamins__see_Medium_No__197__["Trace vitamins (see Medium No. 197 )"]
CultureMech_015864 --> ing9_Trace_vitamins__see_Medium_No__197__
ing10_Distilled_water["Distilled water"]
CultureMech_015864 --> ing10_Distilled_water
ing10_Distilled_water -.-> CHEBI_15377
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | HEPES | 23.8 G_PER_L |
CHEBI:46756
HEPES
|
|
| 2 | Peptone | 0.25 G_PER_L | ||
| 3 | Yeast extract (BD-Difco) | 0.25 G_PER_L | ||
| 4 | Wolfe's mineral solution (see Medium No. 265 ) | 1.0 ML_PER_L | ||
| 5 | Modified Hutner's basal salts (see Medium No. 900 ) | 20.0 ML_PER_L | ||
| 6 | Artificial seawater | 250.0 ML_PER_L | ||
| 7 |
Distilled water
H2O 18.015 g/mol
|
729.0 ML_PER_L |
CHEBI:15377
water
|
|
| 8 |
Glucose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17234
glucose
|
|
| 9 | N -Acetyl-D-glucosamine | 1.0 G_PER_L | ||
| 10 | Trace vitamins (see Medium No. 197 ) | 1.0 ML_PER_L | ||
| 11 |
Distilled water
H2O 18.015 g/mol
|
9.0 ML_PER_L |
CHEBI:15377
water
|
Preparation
- Adjust pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1429_M1H_NAG_ASW (CultureMech:015864)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-02T07:09:14.733357+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1429. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-06-03T02:00:03.353443Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-06-03T07:12:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-08-06T03:04:35.625535+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 424b38c3a27ad9dc52a5c8052f3d07ab4a42ca84af1806447bf9b797e3b197a3 |