desulfosporosinus_sb140_medium

Overview

External ontology

jcm.grmd:1444 (source link unavailable) — DESULFOSPOROSINUS SB140 MEDIUM

Applications

• Microbial cultivation

pH

7.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1444

Composition

flowchart LR
    CultureMech_015868["desulfosporosinus_sb140_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_015868 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_015868 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_7H2O["MgSO4-7H2O"]
    CultureMech_015868 --> ing2_MgSO4_7H2O
    ing3_KCl["KCl"]
    CultureMech_015868 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_015868 --> ing4_Yeast_extract
    ing5_Ca_NO3_2_4H2O["Ca(NO3)2•4H2O"]
    CultureMech_015868 --> ing5_Ca_NO3_2_4H2O
    ing6_Na2SO4["Na2SO4"]
    CultureMech_015868 --> ing6_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing6_Na2SO4 -.-> CHEBI_32149
    ing7_Trace_element_solution__see_Medium_No__439__["Trace element solution (see Medium No. 439 )"]
    CultureMech_015868 --> ing7_Trace_element_solution__see_Medium_No__439__
    ing8_L_Cysteine_HCl_H2O["L-Cysteine•HCl•H2O"]
    CultureMech_015868 --> ing8_L_Cysteine_HCl_H2O
    ing9_Resazurin["Resazurin"]
    CultureMech_015868 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Distilled_water["Distilled water"]
    CultureMech_015868 --> ing10_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing10_Distilled_water -.-> CHEBI_15377
    ing11_5__Na2HPO4_solution["5% Na2HPO4 solution"]
    CultureMech_015868 --> ing11_5__Na2HPO4_solution
    ing12_5__KH2PO4_solution["5% KH2PO4 solution"]
    CultureMech_015868 --> ing12_5__KH2PO4_solution
    ing13_1M_Glycerin_solution["1M Glycerin solution"]
    CultureMech_015868 --> ing13_1M_Glycerin_solution
    ing14_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
    CultureMech_015868 --> ing14_Trace_vitamins___see_Medium_No__197__

Ingredients (15)

Preparation

1. Mix components thoroughly, adjust pH to 7.0, and autoclave under a N2-CO2 (4:1, v/v) gas mixture.
2. After cooling, aseptically and anaerobically add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J1444_DESULFOSPOROSINUS_SB140_MEDIUM (CultureMech:015868)