pmx_108_medium

Overview

External ontology

jcm.grmd:1445 (source link unavailable) — PMX.108 MEDIUM

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1445

Composition

flowchart LR
    CultureMech_015869["pmx_108_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_015869 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_015869 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgCl2_6H2O["MgCl2•6H2O"]
    CultureMech_015869 --> ing2_MgCl2_6H2O
    ing3_CaCl2_2H2O["CaCl2•2H2O"]
    CultureMech_015869 --> ing3_CaCl2_2H2O
    ing4_Sodium_aceate_3H2O["Sodium aceate•3H2O"]
    CultureMech_015869 --> ing4_Sodium_aceate_3H2O
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_015869 --> ing5_Yeast_extract
    ing6_Casamino_acids__BD_Difico_["Casamino acids (BD-Difico)"]
    CultureMech_015869 --> ing6_Casamino_acids__BD_Difico_
    ing7_Tryptone__BD_Difico_["Tryptone (BD-Difico)"]
    CultureMech_015869 --> ing7_Tryptone__BD_Difico_
    ing8_Trace_mineral_solution__see_Medium_No__852__["Trace mineral solution (see Medium No. 852 )"]
    CultureMech_015869 --> ing8_Trace_mineral_solution__see_Medium_No__852__
    ing9_Se_W_solution__see_below_["Se/W solution (see below)"]
    CultureMech_015869 --> ing9_Se_W_solution__see_below_
    ing10_Trace_vitamins_solution__see_Medium_No__284__["Trace vitamins solution (see Medium No. 284 )"]
    CultureMech_015869 --> ing10_Trace_vitamins_solution__see_Medium_No__284__
    ing11_Resazurin["Resazurin"]
    CultureMech_015869 --> ing11_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing11_Resazurin -.-> CHEBI_8806
    ing12_Distilled_water["Distilled water"]
    CultureMech_015869 --> ing12_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing12_Distilled_water -.-> CHEBI_15377
    ing13_8__NaHCO3_solution_["8% NaHCO3 solution*"]
    CultureMech_015869 --> ing13_8__NaHCO3_solution_
    ing14_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine•HCl•H2O solution"]
    CultureMech_015869 --> ing14_5__L_Cysteine_HCl_H2O_solution
    ing15_5__Na2S_9H2O_solution["5% Na2S•9H2O solution"]
    CultureMech_015869 --> ing15_5__Na2S_9H2O_solution
    ing16_Na2SeO3["Na2SeO3"]
    CultureMech_015869 --> ing16_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing16_Na2SeO3 -.-> CHEBI_48843
    ing17_Na2WO4_2H2O["Na2WO4•2H2O"]
    CultureMech_015869 --> ing17_Na2WO4_2H2O
    ing18_Distilled_water["Distilled water"]
    CultureMech_015869 --> ing18_Distilled_water
    ing18_Distilled_water -.-> CHEBI_15377

Ingredients (19)

Preparation

1. Mix components thoroughly, bring to a boil, and cool down under a N2-CO2 (4:1, v/v) gas stream.
2. Distribute the medium into culture vessels under the same gas mixture (e. g., 20 ml medium in 60 ml serum bottles), seal with butyl rubber stoppers and inject 10-20% gas-phase volume of H2, and autoclave.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J1445_PMX_108_MEDIUM (CultureMech:015869)