freshwater_thiosulfate_oxidizing_bacteria_medium

Overview

External ontology

jcm.grmd:1347 (source link unavailable) — FRESHWATER THIOSULFATE-OXIDIZING BACTERIA MEDIUM

Applications

• Microbial cultivation

pH

6.5

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1347

Composition

flowchart LR
    CultureMech_015883["freshwater_thiosulfate_oxidizing_bacteria_medium"]
    ing0_Modified_Wolfe_s_solution__see_Medium_No__628__["Modified Wolfe's solution (see Medium No. 628 )"]
    CultureMech_015883 --> ing0_Modified_Wolfe_s_solution__see_Medium_No__628__
    ing1_Trace_minerals__see_Medium_No__151__["Trace minerals (see Medium No. 151 )"]
    CultureMech_015883 --> ing1_Trace_minerals__see_Medium_No__151__

Ingredients (2)

Preparation

1. Mix components and autoclave.
2. Aseptically add per liter the following solutions from sterile stocks (autoclaved or *filter-sterilized): Check final pH to be 6.0-6.5.
3. Dispense into culture vessels, replace the gas phase with a N2-CO2-O2 (79:20:1, v/v/v) gas mixture, and seal with butyl rubber stoppers.
4. Modified Wolfe's Mineral solution: Trace minerals: Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH.
5. Then proceed to add minerals.
6. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J1347_FRESHWATER_THIOSULFATE_OXIDIZING_BACTERIA_MEDIUM (CultureMech:015883)