freshwater_thiosulfate_oxidizing_bacteria_medium

Overview

External ontology

jcm.grmd:1347 (source link unavailable) — FRESHWATER THIOSULFATE-OXIDIZING BACTERIA MEDIUM

Applications

• Microbial cultivation

pH

6.5

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1347

Composition

flowchart LR
    CultureMech_015883["freshwater_thiosulfate_oxidizing_bacteria_medium"]
    ing0_Modified_Wolfe_s_solution__see_Medium_No__628__["Modified Wolfe's solution (see Medium No. 628 )"]
    CultureMech_015883 --> ing0_Modified_Wolfe_s_solution__see_Medium_No__628__
    ing1_Trace_minerals__see_Medium_No__151__["Trace minerals (see Medium No. 151 )"]
    CultureMech_015883 --> ing1_Trace_minerals__see_Medium_No__151__

Ingredients (2)

Preparation

1. Mix components and autoclave.
2. Aseptically add per liter the following solutions from sterile stocks (autoclaved or *filter-sterilized): Check final pH to be 6.0-6.5.
3. Dispense into culture vessels, replace the gas phase with a N2-CO2-O2 (79:20:1, v/v/v) gas mixture, and seal with butyl rubber stoppers.
4. Modified Wolfe's Mineral solution: Trace minerals: Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH.
5. Then proceed to add minerals.
6. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance