Overview
- External ontology
- jcm.grmd:1367 (source link unavailable) — MARINE DESULFOVIBRIO COMPLEX MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1367
Composition
flowchart LR
CultureMech_015885["marine_desulfovibrio_complex_medium"]
ing0_Sea_salts__Sigma_["Sea salts (Sigma)"]
CultureMech_015885 --> ing0_Sea_salts__Sigma_
ing1_Wolfe_s_mineral_elixir__see_Medium_No__470__["Wolfe's mineral elixir (see Medium No. 470 )"]
CultureMech_015885 --> ing1_Wolfe_s_mineral_elixir__see_Medium_No__470__
ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_015885 --> ing2_Yeast_extract__BD_Difco_
ing3_Resazurin["Resazurin"]
CultureMech_015885 --> ing3_Resazurin
ing4_Distilled_water["Distilled water"]
CultureMech_015885 --> ing4_Distilled_water
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea salts (Sigma) | 35.0 G_PER_L | ||
| 2 | Wolfe's mineral elixir (see Medium No. 470 ) | 1.0 ML_PER_L | ||
| 3 | Yeast extract (BD-Difco) | 1.0 G_PER_L | ||
| 4 | Resazurin | 0.5 MG_PER_L | ||
| 5 | Distilled water | 945.0 ML_PER_L |
Preparation
- Mix components thoroughly and autoclave under a N2 atmosphere.
- Aseptically and anaerobically add the following solutions from sterile anaerobic stock solutions (autoclaved or *filter-sterilized): Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a stream of N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1367_MARINE_DESULFOVIBRIO_COMPLEX_MEDIUM (CultureMech:015885)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-01T01:54:22.709791+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1367. Ingredients captured verbatim. CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-10-01T02:55:00+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015885 |
| 2026-10-01T03:00:53.939403+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ae3ff6f5fdd77e19e89aa4ccdda55eeee66e6d00efc66d67fe0f6180b14857a8 |