marine_desulfovibrio_complex_medium

Overview

External ontology

jcm.grmd:1367 (source link unavailable) — MARINE DESULFOVIBRIO COMPLEX MEDIUM

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1367

Composition

flowchart LR
    CultureMech_015885["marine_desulfovibrio_complex_medium"]
    ing0_Sea_salts__Sigma_["Sea salts (Sigma)"]
    CultureMech_015885 --> ing0_Sea_salts__Sigma_
    ing1_Wolfe_s_mineral_elixir__see_Medium_No__470__["Wolfe's mineral elixir (see Medium No. 470 )"]
    CultureMech_015885 --> ing1_Wolfe_s_mineral_elixir__see_Medium_No__470__
    ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_015885 --> ing2_Yeast_extract__BD_Difco_
    ing3_Resazurin["Resazurin"]
    CultureMech_015885 --> ing3_Resazurin
    ing4_Distilled_water["Distilled water"]
    CultureMech_015885 --> ing4_Distilled_water

Ingredients (5)

Preparation

1. Mix components thoroughly and autoclave under a N2 atmosphere.
2. Aseptically and anaerobically add the following solutions from sterile anaerobic stock solutions (autoclaved or *filter-sterilized): Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a stream of N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers.

Target organisms

No target organisms are recorded.

Provenance